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Sample GSM2335321 Query DataSets for GSM2335321
Status Public on Apr 04, 2017
Title 17-UC-L5-4
Sample type RNA
 
Source name dorsolateral prefrontal cortex
Organism Homo sapiens
Characteristics tissue: dorsolateral prefrontal cortex
cell type: pyramidal neuron cell
shape: characteristic triangular shape and prominent apical dendrite
genotype: Unaffected Comparison subject
dsm-iv diagnoses: Unaffected Comparison
Treatment protocol An independent committee of experienced research clinicians made consensus DSM-IV diagnoses for each subject using information obtained from medical records and structured diagnostic interviews conducted with the decedent’s family members.
Growth protocol Brain specimens were obtained during autopsies conducted at the Allegheny County Office of the Medical Examiner (Pittsburgh, PA) after consent was obtained from the next-of-kin.
Extracted molecule total RNA
Extraction protocol For each sample, RNA was extracted using the QIAGEN® RNeasy Plus Micro kit (QIAGEN, Valencia).
Label Encore Biotin module (Nugen)
Label protocol Labeled using the Encore Biotin module (Nugen).
 
Hybridization protocol CDNA was synthesized and amplified using the Ovation Pico WTA System.
Scan protocol Loaded on an Affymetrix GeneChip® U219.
Description Gene expression data for subject 17, diagnosis UC, layer 5, replicate 2
Data processing Expression intensities were extracted from Affymetrix Expression Console and normalized using Robust Multi-array Average Express.
 
Submission date Oct 04, 2016
Last update date Apr 04, 2017
Contact name David Lewis
E-mail(s) lewisda@upmc.edu
Organization name University of Pittsburgh
Department Psychiatry and Neuroscience
Street address 3811 O'Hara St
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
 
Platform ID GPL13667
Series (1)
GSE87610 Gene expression of L3 and L5 pyramidal neurons in the DLPFC comparing schizophrenia from bipolar major depressive disorders and unaffected subjects.

Data table header descriptions
ID_REF
VALUE Background corrected, log2 transformed and then quantile normalized gene expression intensity.

Data table
ID_REF VALUE
AFFX-DapX-5_at 2.80201
AFFX-DapX-M_at 2.287196
AFFX-DapX-3_at 2.313199
AFFX-LysX-5_at 2.021515
AFFX-LysX-M_at 2.479104
AFFX-LysX-3_at 2.492022
AFFX-PheX-5_at 2.165357
AFFX-PheX-M_at 2.595743
AFFX-PheX-3_at 2.780754
AFFX-ThrX-5_at 2.343032
AFFX-ThrX-M_at 2.168963
AFFX-ThrX-3_at 2.813515
AFFX-TrpnX-5_at 2.205154
AFFX-TrpnX-M_at 2.375507
AFFX-TrpnX-3_at 2.305636
AFFX-r2-Ec-bioB-5_at 7.898787
AFFX-r2-Ec-bioB-M_at 8.181081
AFFX-r2-Ec-bioB-3_at 8.004775
AFFX-r2-Ec-bioC-5_at 9.220654
AFFX-r2-Ec-bioC-3_at 8.770487

Total number of rows: 49386

Table truncated, full table size 1081 Kbytes.




Supplementary file Size Download File type/resource
GSM2335321_5500994192207072414569_C10.cel.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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