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Sample GSM2335262 Query DataSets for GSM2335262
Status Public on Apr 04, 2017
Title 68-SZ-L3-1
Sample type RNA
 
Source name dorsolateral prefrontal cortex
Organism Homo sapiens
Characteristics tissue: dorsolateral prefrontal cortex
cell type: pyramidal neuron cell
shape: characteristic triangular shape and prominent apical dendrite
genotype: Schizophrenia
dsm-iv diagnoses: DS
Treatment protocol An independent committee of experienced research clinicians made consensus DSM-IV diagnoses for each subject using information obtained from medical records and structured diagnostic interviews conducted with the decedent’s family members.
Growth protocol Brain specimens were obtained during autopsies conducted at the Allegheny County Office of the Medical Examiner (Pittsburgh, PA) after consent was obtained from the next-of-kin.
Extracted molecule total RNA
Extraction protocol For each sample, RNA was extracted using the QIAGEN® RNeasy Plus Micro kit (QIAGEN, Valencia).
Label Encore Biotin module (Nugen)
Label protocol Labeled using the Encore Biotin module (Nugen).
 
Hybridization protocol CDNA was synthesized and amplified using the Ovation Pico WTA System.
Scan protocol Loaded on an Affymetrix GeneChip® U219.
Description Gene expression data for subject 68, diagnosis SZ, layer 3, replicate 1
Data processing Expression intensities were extracted from Affymetrix Expression Console and normalized using Robust Multi-array Average Express.
 
Submission date Oct 04, 2016
Last update date Apr 04, 2017
Contact name David Lewis
E-mail(s) lewisda@upmc.edu
Organization name University of Pittsburgh
Department Psychiatry and Neuroscience
Street address 3811 O'Hara St
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
 
Platform ID GPL13667
Series (1)
GSE87610 Gene expression of L3 and L5 pyramidal neurons in the DLPFC comparing schizophrenia from bipolar major depressive disorders and unaffected subjects.

Data table header descriptions
ID_REF
VALUE Background corrected, log2 transformed and then quantile normalized gene expression intensity.

Data table
ID_REF VALUE
AFFX-DapX-5_at 2.582787
AFFX-DapX-M_at 2.18679
AFFX-DapX-3_at 2.132667
AFFX-LysX-5_at 2.182182
AFFX-LysX-M_at 2.266176
AFFX-LysX-3_at 2.221559
AFFX-PheX-5_at 2.029866
AFFX-PheX-M_at 2.261352
AFFX-PheX-3_at 2.568547
AFFX-ThrX-5_at 2.310325
AFFX-ThrX-M_at 2.15558
AFFX-ThrX-3_at 2.921557
AFFX-TrpnX-5_at 2.160902
AFFX-TrpnX-M_at 2.509355
AFFX-TrpnX-3_at 2.194239
AFFX-r2-Ec-bioB-5_at 7.509885
AFFX-r2-Ec-bioB-M_at 7.604496
AFFX-r2-Ec-bioB-3_at 7.65202
AFFX-r2-Ec-bioC-5_at 8.872089
AFFX-r2-Ec-bioC-3_at 8.586491

Total number of rows: 49386

Table truncated, full table size 1081 Kbytes.




Supplementary file Size Download File type/resource
GSM2335262_5500994180309022414523_A10.cel.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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