|
| Status |
Public on Apr 04, 2017 |
| Title |
50-MDD-L3-2 |
| Sample type |
RNA |
| |
|
| Source name |
dorsolateral prefrontal cortex
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: dorsolateral prefrontal cortex
cell type: pyramidal neuron cell
shape: characteristic triangular shape and prominent apical dendrite
genotype: Major Depressive Disorder
dsm-iv diagnoses: MDD, recurrent
|
| Treatment protocol |
An independent committee of experienced research clinicians made consensus DSM-IV diagnoses for each subject using information obtained from medical records and structured diagnostic interviews conducted with the decedent’s family members.
|
| Growth protocol |
Brain specimens were obtained during autopsies conducted at the Allegheny County Office of the Medical Examiner (Pittsburgh, PA) after consent was obtained from the next-of-kin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For each sample, RNA was extracted using the QIAGEN® RNeasy Plus Micro kit (QIAGEN, Valencia).
|
| Label |
Encore Biotin module (Nugen)
|
| Label protocol |
Labeled using the Encore Biotin module (Nugen).
|
| |
|
| Hybridization protocol |
CDNA was synthesized and amplified using the Ovation Pico WTA System.
|
| Scan protocol |
Loaded on an Affymetrix GeneChip® U219.
|
| Description |
Gene expression data for subject 50, diagnosis MDD, layer 3, replicate 2
|
| Data processing |
Expression intensities were extracted from Affymetrix Expression Console and normalized using Robust Multi-array Average Express.
|
| |
|
| Submission date |
Oct 04, 2016 |
| Last update date |
Apr 04, 2017 |
| Contact name |
David Lewis |
| E-mail(s) |
lewisda@upmc.edu
|
| Organization name |
University of Pittsburgh
|
| Department |
Psychiatry and Neuroscience
|
| Street address |
3811 O'Hara St
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE87610 |
Gene expression of L3 and L5 pyramidal neurons in the DLPFC comparing schizophrenia from bipolar major depressive disorders and unaffected subjects. |
|
