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Sample GSM2335218 Query DataSets for GSM2335218
Status Public on Apr 04, 2017
Title 65-SZ-L5-4
Sample type RNA
 
Source name dorsolateral prefrontal cortex
Organism Homo sapiens
Characteristics tissue: dorsolateral prefrontal cortex
cell type: pyramidal neuron cell
shape: characteristic triangular shape and prominent apical dendrite
genotype: Schizophrenia
dsm-iv diagnoses: US; ADC; OAC
Treatment protocol An independent committee of experienced research clinicians made consensus DSM-IV diagnoses for each subject using information obtained from medical records and structured diagnostic interviews conducted with the decedent’s family members.
Growth protocol Brain specimens were obtained during autopsies conducted at the Allegheny County Office of the Medical Examiner (Pittsburgh, PA) after consent was obtained from the next-of-kin.
Extracted molecule total RNA
Extraction protocol For each sample, RNA was extracted using the QIAGEN® RNeasy Plus Micro kit (QIAGEN, Valencia).
Label Encore Biotin module (Nugen)
Label protocol Labeled using the Encore Biotin module (Nugen).
 
Hybridization protocol CDNA was synthesized and amplified using the Ovation Pico WTA System.
Scan protocol Loaded on an Affymetrix GeneChip® U219.
Description Gene expression data for subject 65, diagnosis SZ, layer 5, replicate 2
Data processing Expression intensities were extracted from Affymetrix Expression Console and normalized using Robust Multi-array Average Express.
 
Submission date Oct 04, 2016
Last update date Apr 04, 2017
Contact name David Lewis
E-mail(s) lewisda@upmc.edu
Organization name University of Pittsburgh
Department Psychiatry and Neuroscience
Street address 3811 O'Hara St
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
 
Platform ID GPL13667
Series (1)
GSE87610 Gene expression of L3 and L5 pyramidal neurons in the DLPFC comparing schizophrenia from bipolar major depressive disorders and unaffected subjects.

Data table header descriptions
ID_REF
VALUE Background corrected, log2 transformed and then quantile normalized gene expression intensity.

Data table
ID_REF VALUE
AFFX-DapX-5_at 2.630699
AFFX-DapX-M_at 2.395688
AFFX-DapX-3_at 2.289664
AFFX-LysX-5_at 2.068521
AFFX-LysX-M_at 2.600916
AFFX-LysX-3_at 2.579307
AFFX-PheX-5_at 2.577175
AFFX-PheX-M_at 2.816335
AFFX-PheX-3_at 2.298476
AFFX-ThrX-5_at 2.266207
AFFX-ThrX-M_at 2.641767
AFFX-ThrX-3_at 2.810656
AFFX-TrpnX-5_at 2.198235
AFFX-TrpnX-M_at 2.328971
AFFX-TrpnX-3_at 2.49729
AFFX-r2-Ec-bioB-5_at 8.195906
AFFX-r2-Ec-bioB-M_at 8.36086
AFFX-r2-Ec-bioB-3_at 8.251347
AFFX-r2-Ec-bioC-5_at 9.436266
AFFX-r2-Ec-bioC-3_at 9.191448

Total number of rows: 49386

Table truncated, full table size 1081 Kbytes.




Supplementary file Size Download File type/resource
GSM2335218_5500994180309022414523_H01.cel.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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