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Sample GSM2335154 Query DataSets for GSM2335154
Status Public on Apr 04, 2017
Title 6-UC-L5-4
Sample type RNA
 
Source name dorsolateral prefrontal cortex
Organism Homo sapiens
Characteristics tissue: dorsolateral prefrontal cortex
cell type: pyramidal neuron cell
shape: characteristic triangular shape and prominent apical dendrite
genotype: Unaffected Comparison subject
dsm-iv diagnoses: Unaffected Comparison
Treatment protocol An independent committee of experienced research clinicians made consensus DSM-IV diagnoses for each subject using information obtained from medical records and structured diagnostic interviews conducted with the decedent’s family members.
Growth protocol Brain specimens were obtained during autopsies conducted at the Allegheny County Office of the Medical Examiner (Pittsburgh, PA) after consent was obtained from the next-of-kin.
Extracted molecule total RNA
Extraction protocol For each sample, RNA was extracted using the QIAGEN® RNeasy Plus Micro kit (QIAGEN, Valencia).
Label Encore Biotin module (Nugen)
Label protocol Labeled using the Encore Biotin module (Nugen).
 
Hybridization protocol CDNA was synthesized and amplified using the Ovation Pico WTA System.
Scan protocol Loaded on an Affymetrix GeneChip® U219.
Description Gene expression data for subject 6, diagnosis UC, layer 5, replicate 2
Data processing Expression intensities were extracted from Affymetrix Expression Console and normalized using Robust Multi-array Average Express.
 
Submission date Oct 04, 2016
Last update date Apr 04, 2017
Contact name David Lewis
E-mail(s) lewisda@upmc.edu
Organization name University of Pittsburgh
Department Psychiatry and Neuroscience
Street address 3811 O'Hara St
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
 
Platform ID GPL13667
Series (1)
GSE87610 Gene expression of L3 and L5 pyramidal neurons in the DLPFC comparing schizophrenia from bipolar major depressive disorders and unaffected subjects.

Data table header descriptions
ID_REF
VALUE Background corrected, log2 transformed and then quantile normalized gene expression intensity.

Data table
ID_REF VALUE
AFFX-DapX-5_at 2.659584
AFFX-DapX-M_at 2.340861
AFFX-DapX-3_at 2.398329
AFFX-LysX-5_at 2.14317
AFFX-LysX-M_at 2.597723
AFFX-LysX-3_at 2.857885
AFFX-PheX-5_at 2.28559
AFFX-PheX-M_at 2.513467
AFFX-PheX-3_at 2.690801
AFFX-ThrX-5_at 2.489237
AFFX-ThrX-M_at 2.549664
AFFX-ThrX-3_at 3.503249
AFFX-TrpnX-5_at 2.179894
AFFX-TrpnX-M_at 2.618186
AFFX-TrpnX-3_at 2.53867
AFFX-r2-Ec-bioB-5_at 7.955031
AFFX-r2-Ec-bioB-M_at 8.357642
AFFX-r2-Ec-bioB-3_at 8.018815
AFFX-r2-Ec-bioC-5_at 9.416048
AFFX-r2-Ec-bioC-3_at 9.097527

Total number of rows: 49386

Table truncated, full table size 1081 Kbytes.




Supplementary file Size Download File type/resource
GSM2335154_5500994180309022414523_G06.cel.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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