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Sample GSM2332924 Query DataSets for GSM2332924
Status Public on Mar 16, 2017
Title 2xlink IgG ChIP-seq rep 2, 3-13-14
Sample type SRA
Source name S2_2xlink IgG ChIP-seq
Organism Drosophila melanogaster
Characteristics cell line: S2 cells
chip antibody: Normal rabbit IgG
chip antibody information: Cell Signaling, catalog number 2729
crosslinker: 2mM ethylene glycol bis(succinimidyl succinate) and 1% formaldehyde
Treatment protocol Crosslinked with ethylene glycol bis(succinimidyl succinate) and then crosslinked with formaldehyde
Extracted molecule genomic DNA
Extraction protocol Cells were sonicated and cleared of cellular debris. Lysates were immunoprecipitated with the corresponding antibodies
Briefly, DNA ends were repaired with T4 DNA polymerase, Klenow Large Fragment DNA polyermerase and T4 polynclease kinase. DNA was purified with PEG bead slurry. Illumina short sequencing adaptor was ligated to DNA overnight. Library was purified with PEG beads
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina MiSeq
Description bam file was generated from fastq files
Data processing Base calling done with MiSeq (Illumina RTA version 1.18.54)
Reads aligned with BWA (v0.6.1), done using default parameters
Genome_build: BDGP release 5 (dm3)
Supplementary_files_format_and_content: wig files were generated from bam files
Submission date Sep 30, 2016
Last update date May 15, 2019
Contact name Helen McNeill
Organization name Lunenfeld Tanenbaum Research Institute
Street address 600 University Ave.
City Toronto
State/province Ontario
ZIP/Postal code M5G1X5
Country Canada
Platform ID GPL16479
Series (1)
GSE87509 ChIP-seq of Atrophin in Drosophila S2 cells
BioSample SAMN05853730
SRA SRX2200903

Supplementary file Size Download File type/resource
GSM2332924_IgG-3-13-14_S4.bam.q5.F1028.PET.wig.gz 4.9 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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