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Sample GSM2330013 Query DataSets for GSM2330013
Status Public on Sep 28, 2017
Title SubjectID 64, high MC-SFA, before
Sample type RNA
 
Source name subcutaneous adipose tissue, high MC-SFA, before
Organism Homo sapiens
Characteristics gender: male
individual: 64
diet_group: high MC-SFA + casein
tissue: subcutaneous adipose tissue
metabolic syndrome: n
age: 65
weight: 99
height: 183.2
waisttohipratio: 1.02
Treatment protocol This study is a secondary analysis of a randomized, parallel-controlled, double-blinded, 12-wk diet intervention study that was designed to examine the effects of milk fat, with a high or low MC-SFA content, and of milk protein, whey or casein, on post-prandial lipemia. Design and methods of the original study were described in detail previously (Bohl et al. 2015, doi: 10.3945/ajcn.114.097923). Briefly, 52 participants were randomized in a 2 × 2 factorial design to one of 4 diets: high MC-SFA + casein, low MC-SFA + casein, high MC-SFA + whey, and low MC-SFA + whey. In this paper, we examine the effects of MC-SFAs on whole genome gene expression in subcutaneous adipose tissue in a subpopulation of 12 subjects, 6 in the high MC-SFA + casein group and 6 in the low MC-SFA + casein groups.
Growth protocol Participants needed to be ≥18 y of age, have an abdominal circumference of ≥80 cm for women or ≥94 cm for men, and be weight stabile for at least 3 months prior to inclusion. Furthermore, they could not be diabetic, pregnant, lactating, or suffer from severe cardiovascular, renal, endocrine, or psychiatric disease. All participants gave written informed consent to participate in the study and the study protocol was approved by the Central Denmark Region Committees on Health Research Ethics. The study was registered at clinicaltrials.gov as NCT01472666.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using RNeasy micro kit from Qiagen. RNA integrity was checked on chip analysis (Agilent 2100 bioanalyzer, Agilent Technologies, Amsterdam, the Netherlands) according to the manufacturer's instructions. RNA was judged as suitable for array hybridization only if samples exhibited intact bands corresponding to the 18S and 28S ribosomal RNA subunits, and displayed no chromosomal peaks or RNA degradation products (RNA Integrity Number > 7.0).
Label biotin
Label protocol One hundred nanogram of RNA was used for whole transcript cDNA synthesis with the Ambion WT expression kit [catalog number 4411974] (Applied Biosystems/Life Technologies, Nieuwekerk a/d IJssel, The Netherlands).
 
Hybridization protocol Hybridization and washing of the Affymetrix GeneChip Human Gene 1.1 ST arrays were performed on a GeneTitan Instrument (Affymetrix, Santa Clara, CA) according to the manufacturer's recommendations.
Scan protocol Arrays were scanned on an Affymetrix GeneTitan instrument (Affymetrix, Santa Clara, CA).
Description G144_E05_64_m1t0_mn4
Data processing Expression estimates were calculated applying the RMA algorithm in the Bioconductor library 'Oligo' (v1.34.2).
 
Submission date Sep 27, 2016
Last update date Sep 28, 2017
Contact name Juri C Matualatupauw
E-mail(s) jurimatualatupauw@gmail.com
Organization name Wageningen University
Department Human Nutrition
Street address PO Box 17
City Wageningen
ZIP/Postal code 6700 AA
Country Netherlands
 
Platform ID GPL11532
Series (1)
GSE87382 Dietary medium-chain saturated fatty acids induce gene expression of energy metabolism-related pathways in adipose tissue of abdominally obese subjects

Data table header descriptions
ID_REF
VALUE RMA signal (as log2)

Data table
ID_REF VALUE
7892501 1.402700752
7892502 3.18958829
7892503 2.458638077
7892504 7.543415781
7892505 2.096893152
7892506 3.828443465
7892507 4.656770119
7892508 6.083335121
7892509 9.075506891
7892510 5.113537009
7892511 3.386699724
7892512 4.382463751
7892513 2.073735668
7892514 8.657131282
7892515 8.039444673
7892516 2.486921969
7892517 5.512484966
7892518 2.603424394
7892519 2.245601445
7892520 7.585378751

Total number of rows: 33297

Table truncated, full table size 646 Kbytes.




Supplementary file Size Download File type/resource
GSM2330013_G144_E05_64_m1t0_mn4.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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