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Sample GSM2310608 Query DataSets for GSM2310608
Status Public on Sep 15, 2016
Title LNCaP(BHC80-2)_3
Sample type RNA
 
Source name LNCaP(BHC80-2)
Organism Homo sapiens
Characteristics tumor subtype: Adenocarcinoma
cell line: LNCaP
treatment: BHC80-2 overexpression
Extracted molecule total RNA
Extraction protocol RNA extraction protocol not provided.
Label Cy3
Label protocol The quality of the RNA samples was checked with the Agilent 2100 Bioanalyzer and NanoDrop ND-2000 UV-VIS spectrophotometer. Only samples with RNA Integrity Number (RIN) ≥8.0, A260/280 OD values between 1.8 and 2.0 and an A260/A230 OD value of 2.0 were used for one-color labelling using Agilent’s One-Colour Microarray-Based Gene Expression Analysis Low Input Quick Amp Labelling v6.0 (Agilent Technologies, Santa Clara, CA). Total RNA (100 ng) was used to generate cyanine-3-labeled cRNA.
 
Hybridization protocol cRNAs were hybridized on Agilent SurePrint G3 Human Gene Expression v2 8x60K Microarray (AMDID 039494).
Scan protocol Arrays were scanned with an Agilent DNA Microarray Scanner at a 3 µm scan resolution
Description SAMPLE 9
Data processing Data were processed with Agilent Feature Extraction 11.0.1.1. Processed signals were quantile normalized with Agilent GeneSpring 12.0. Raw and normalized data can also be found in the file AgilentHumanv2GE_20Nov14.txt available on the series record.
 
Submission date Sep 14, 2016
Last update date Apr 23, 2018
Contact name Shawn Anderson
E-mail(s) Sanderson@prostatecentre.com
Organization name Vancouver Prostate Centre
Lab Laboratory for Advanced Genome Analysis
Street address 2660 Oak Street
City Vancouver
State/province BC
ZIP/Postal code V6H3Z6
Country Canada
 
Platform ID GPL17077
Series (1)
GSE86942 RNA Splicing of the BHC80 gene Facilitates Neuroendocrine Prostate Cancer Progression
Relations
Reanalyzed by GSE113533

Data table header descriptions
ID_REF
VALUE quantile normalized signal

Data table
ID_REF VALUE
GE_BrightCorner 16.349564
DarkCorner 1.8280972
A_23_P117082 12.987076
A_33_P3246448 1.902826
A_33_P3318220 1.8990911
A_33_P3236322 1.8955867
A_33_P3319925 3.946642
A_21_P0000509 17.945034
A_21_P0000744 11.77644
A_24_P215804 9.146909
A_23_P110167 14.280467
A_33_P3211513 7.2885756
A_23_P103349 2.8366578
A_32_P61480 5.9018483
A_33_P3788124 1.8440274
A_33_P3414202 7.2529182
A_33_P3316686 6.856979
A_33_P3300975 8.562755
A_33_P3263061 13.282759
A_33_P3261373 1.8124344

Total number of rows: 50739

Table truncated, full table size 1148 Kbytes.




Supplementary file Size Download File type/resource
GSM2310608_US09503746_253949437531_S01_GE1_1100_Jul11_1_4.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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