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Sample GSM2310601 Query DataSets for GSM2310601
Status Public on Sep 15, 2016
Title LNCaP(control)_2
Sample type RNA
Source name LNCaP(control)
Organism Homo sapiens
Characteristics tumor subtype: Adenocarcinoma
cell line: LNCaP
treatment: control overexpression
Extracted molecule total RNA
Extraction protocol RNA extraction protocol not provided.
Label Cy3
Label protocol The quality of the RNA samples was checked with the Agilent 2100 Bioanalyzer and NanoDrop ND-2000 UV-VIS spectrophotometer. Only samples with RNA Integrity Number (RIN) ≥8.0, A260/280 OD values between 1.8 and 2.0 and an A260/A230 OD value of 2.0 were used for one-color labelling using Agilent’s One-Colour Microarray-Based Gene Expression Analysis Low Input Quick Amp Labelling v6.0 (Agilent Technologies, Santa Clara, CA). Total RNA (100 ng) was used to generate cyanine-3-labeled cRNA.
Hybridization protocol cRNAs were hybridized on Agilent SurePrint G3 Human Gene Expression v2 8x60K Microarray (AMDID 039494).
Scan protocol Arrays were scanned with an Agilent DNA Microarray Scanner at a 3 µm scan resolution
Description SAMPLE 2
Data processing Data were processed with Agilent Feature Extraction Processed signals were quantile normalized with Agilent GeneSpring 12.0. Raw and normalized data can also be found in the file AgilentHumanv2GE_20Nov14.txt available on the series record.
Submission date Sep 14, 2016
Last update date Sep 15, 2016
Contact name Shawn Anderson
Organization name Vancouver Prostate Centre
Lab Laboratory for Advanced Genome Analysis
Street address 2660 Oak Street
City Vancouver
State/province BC
ZIP/Postal code V6H3Z6
Country Canada
Platform ID GPL17077
Series (1)
GSE86942 RNA Splicing of the BHC80 gene Facilitates Neuroendocrine Prostate Cancer Progression

Data table header descriptions
VALUE quantile normalized signal

Data table
GE_BrightCorner 16.628365
DarkCorner 2.0548873
A_23_P117082 12.981156
A_33_P3246448 1.9238927
A_33_P3318220 1.9206175
A_33_P3236322 1.9187118
A_33_P3319925 3.796295
A_21_P0000509 18.142084
A_21_P0000744 11.620997
A_24_P215804 9.402606
A_23_P110167 14.408506
A_33_P3211513 7.9010653
A_23_P103349 2.1800919
A_32_P61480 5.8884277
A_33_P3788124 1.8715632
A_33_P3414202 6.917473
A_33_P3316686 6.805387
A_33_P3300975 10.222611
A_33_P3263061 12.812415
A_33_P3261373 2.9332376

Total number of rows: 50739

Table truncated, full table size 1148 Kbytes.

Supplementary file Size Download File type/resource
GSM2310601_US09503746_253949437532_S01_GE1_1100_Jul11_1_2.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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