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Sample GSM2309303 Query DataSets for GSM2309303
Status Public on Sep 13, 2016
Title Male wild type cardiomyocytes 452
Sample type RNA
 
Source name cardiomyocyte
Organism Mus musculus
Characteristics genotype: wild type
cell type: cardiomyocyte
age: 15 weeks
gender: Male
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from cardiomyocytes using the RNeasy Lipit Tissue Mini Kit (Qiagen Inc) and further purified using RNeasy MinElute Cleanup Kit (Qiagen Inc)
Label Biotin
Label protocol 250 ng total RNA was amplified and labeled according to the Affymetrix 3’-IVT labeling protocol.
 
Hybridization protocol 10 ug biotinylated cRNA was hybridized to the Affymetrix Gene Atlas Mouse MG-430 PM Array Strips according to current Affymetrix protocols.
Scan protocol The arrays were washed and scanned according to standard Affymetrix GeneAtlas instructions using the GeneAtlas scanner and software control (GeneAtlas Instrument Control (GAIC) v. 1.0.5.267.).
Description 452-Male_WT
Data processing Raw data (CEL files) were generated using Affymetrix AGCC software and quantile normalized by the RMA method. Log intensity distributions, pair-wise correlations and internal Affymetrix controls were examined to evaluate hybridization quality using Partek Genomics Suite software (Partek Inc.).
 
Submission date Sep 12, 2016
Last update date Sep 13, 2016
Contact name Hao Wang
E-mail(s) haowang@wakehealth.edu
Organization name Wake Forest School of Medicine
Department Anesthesiology
Street address Medical Center Boulevard
City Winston-Salem
State/province NC
ZIP/Postal code 27157
Country USA
 
Platform ID GPL11180
Series (1)
GSE86843 Expression profiles of the G protein-coupled estrogen receptor (GPER)-knockout versus intact cardiomyocytes

Data table header descriptions
ID_REF
VALUE Normalized log2 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 5.432694
AFFX-BioB-M_at 6.65908
AFFX-BioB-3_at 6.131503
AFFX-BioC-5_at 7.796267
AFFX-BioC-3_at 7.88836
AFFX-BioDn-5_at 9.351234
AFFX-BioDn-3_at 10.35837
AFFX-CreX-5_at 11.5327
AFFX-CreX-3_at 11.59537
AFFX-DapX-5_at 7.059958
AFFX-DapX-M_at 7.912396
AFFX-DapX-3_at 7.689812
AFFX-LysX-5_at 2.739363
AFFX-LysX-M_at 3.53562
AFFX-LysX-3_at 4.282646
AFFX-PheX-5_at 3.27685
AFFX-PheX-M_at 3.688861
AFFX-PheX-3_at 4.477445
AFFX-ThrX-5_at 3.881625
AFFX-ThrX-M_at 4.683777

Total number of rows: 45141

Table truncated, full table size 1027 Kbytes.




Supplementary file Size Download File type/resource
GSM2309303_452-Male_WT.ga.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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