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Sample GSM2308943 Query DataSets for GSM2308943
Status Public on Apr 05, 2017
Title KD1_H3K27ac
Sample type SRA
Source name Endometriosis
Organism Homo sapiens
Characteristics cell type: hTERT immortalized endometriosis cells
genotype/variation: ARID1A Knockdown
antibody: H3K27ac
Extracted molecule genomic DNA
Extraction protocol Nuclei were fixed, lysed, and chromatin was sonicated. Histone-DNA complexes were isolated with antibody. Genomic DNA was isolated by standard phenol-chloroform extraction and ethanol precipitation
Libraries were prepared according to Illumina's instructions and sequenced on either HiSeq-2000 or NextSeq 500 platforms.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
Data processing ChIP-seq reads were aligned to the mm9 genome assembly using EasyAlign version 3.2 with the following configurations…
Non-unique reads were filtered out and reads were mapped to hg19 using BWA
Reads were extended to 200bp, duplicates removed.
Peaks were called using MACS algorithm with a p-value cut-off of 10^-10. Read-count quantification was perfomed then log2 transformed -- corrected for total read count of the largest datastore, only reads within peaks were counted, and corrected for peak length.
Genome_build: hg19
Supplementary_files_format_and_content: Text files with peak list and read-count quantification
Submission date Sep 12, 2016
Last update date May 15, 2019
Contact name Ranjani Lakshminarasimhan
Phone 3238650736
Organization name University of Southern California
Department Urology
Lab Jones/Liang
Street address 1441 Eastlake ave NOR7336
City Los Angeles
State/province California
ZIP/Postal code 90033
Country USA
Platform ID GPL11154
Series (1)
GSE86810 Genome-wide histone modification profiling in endometriosis cells
BioSample SAMN05762547
SRA SRX2158975

Supplementary data files not provided
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

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