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Sample GSM2301176 Query DataSets for GSM2301176
Status Public on Aug 31, 2018
Title Normal adjacent tissue [C171]
Sample type RNA
 
Source name Fresh frozen normal tissue
Organism Homo sapiens
Characteristics molecular subtype: NA
theraoy: Adjuvant
population: Mexico Hispanic
Extracted molecule total RNA
Extraction protocol RNA were extracted from the frozen tissue using the AllPrep DNA/RNA mini kit (Qiagen, Valencia, CA)
Label Biotin
Label protocol cDNA synthesis, amplification, and gene expression profiling were done with the WT PLUS Reagent Kit for fresh samples (Affymetrix) and and labeled with the Genechip hybridization wash and stain kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with Gene Chip Human Transcriptome Array (HTA) 2.0 (Affymetrix) and scanned at the INMEGEN Microarray Core Facility.
Scan protocol Microarrays were read in a scanner and high-resolution model 7Gimage analysis and quality control was performed using the Affymetrix Expression Console software
Description Gene expression data from breast normal adjacent tissue.
Data processing The mRNA expression values were normalized with RMA and quantile algorithms in R with the oligo library from Bioconductor. In order to obtain the intrinsic subtype classification the PAM50 algorithm was performed as described by Parker.
 
Submission date Sep 01, 2016
Last update date Aug 31, 2018
Contact name Sandra Lorena Romero-Cordoba
E-mail(s) sromero_cordoba@hotmail.com
Organization name National Instute of Genomic Medicine
Department Cancer Genomics
Street address Periférico Sur No. 4809, Col. Arenal Tepepan, Delegación Tlalpan. México, D.F.
City Mexico
State/province Mexico
ZIP/Postal code 14610
Country Mexico
 
Platform ID GPL6244
Series (2)
GSE86374 Analysis of somatic DNA copy number alterations and frequency of breast cancer intrinsic subtypes from Mexican women [gene expression]
GSE87049 Analysis of somatic DNA copy number alterations and frequency of breast cancer intrinsic subtypes from Mexican women

Data table header descriptions
ID_REF
VALUE RMA quantile

Data table
ID_REF VALUE
7892501 3.307572504
7892502 5.337661455
7892503 3.458331025
7892504 9.056215102
7892505 3.298519805
7892506 4.40562751
7892507 6.476571145
7892508 7.445248908
7892509 10.85858918
7892510 5.917607254
7892511 3.881745384
7892512 8.404612517
7892513 4.150111562
7892514 10.48171058
7892515 9.599643853
7892516 4.019582544
7892517 6.11293917
7892518 3.159089778
7892519 5.927787854
7892520 10.25066821

Total number of rows: 33297

Table truncated, full table size 646 Kbytes.




Supplementary file Size Download File type/resource
GSM2301176_C171.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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