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Sample GSM2298950 Query DataSets for GSM2298950
Status Public on Feb 13, 2017
Title BS69 ChIP DU145 WT
Sample type SRA
 
Source name brain mets prostate adenocarcinoma
Organism Homo sapiens
Characteristics tissue: prostate adenocarcinoma metastaticized to brain
cancer type: Pca
cell line: DU145
chip target: ZMYND11/BS69
Growth protocol DU145 cells were maintained in DMEM media (Mediatech-Cellgro) with 10% FBS, 100 U/ml Penicillin/Streptomycin, and 5microgram/ml puromycin (if selected)
Extracted molecule genomic DNA
Extraction protocol ChIP DNA was isolated as described in Hollenhorst et. al., Genes and Development, 2011
Sequencing libraries were created as described in Plotnik et.al. Nucleic Acids Research. 2014.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NextSeq 500
 
Data processing Illumina Casava1.8.2 software used for basecalling
Reads were aligned to the Homo sapien genome (UCSC hg19) using Bowtie2 (v2.0.6)
Peak calling was accomplished using the Useq platform (v8.0.1).
Genome_build: UCSC hg19
Supplementary_files_format_and_content: tab-delimited text files include bound regions for each stated sample: chromosome, start, stop
 
Submission date Aug 30, 2016
Last update date May 15, 2019
Contact name Josh Plotnik
E-mail(s) joshua.plotnik@abbvie.com
Organization name AbbVie Inc.
Street address 1 North Waukegan Rd, AP10/210A
City North Chicago
State/province IL
ZIP/Postal code 60064
Country USA
 
Platform ID GPL18573
Series (2)
GSE86238 Interaction with ZMYND11 mediates opposing roles of Ras-responsive transcription factors ETS1 and ETS2
GSE86240 Interaction with ZMYND11 mediates opposing roles of Ras-responsive transcription factors ETS1 and ETS2
Relations
BioSample SAMN05716132
SRA SRX2067986

Supplementary file Size Download File type/resource
GSM2298950_BS69_DU145.txt.gz 5.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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