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Sample GSM2296682 Query DataSets for GSM2296682
Status Public on Feb 09, 2017
Title STAT3 input
Sample type SRA
Source name Bone Marrow Derived Macrophages
Organism Mus musculus
Characteristics genotype: IL10-/-RAG2-/-
strain: 129S6/SvEvTac
cell type: Bone Marrow Derived Macrophage
antibody: STAT3 (Cell Signaling 12640)
Treatment protocol Untreated, IL-10 (1 ng/mL) for 30 min, LPS (1 ng/mL) for 195 min, LPS for 195 min and IL-10 for the last 30 min of the stimulation
Growth protocol Bone marrow precursors were grown in M-CSF conditioned media containing FBS for 5 days prior to stimulation
Extracted molecule genomic DNA
Extraction protocol Macrophages were plated at 2 x 107 cells per condition and after stimulation fixed for 10 minutes in 1% formaldehyde. Chromatin was isolated and sheared by sonication (10 cycles, 20 s on, 30 s rest). After aliquoting an input fraction, 4 x 106 cells were incubated overnight at 4°C with Protein A Dynabeads pre-coupled with 300ng of anti-STAT3 Ab (Cell Signaling 12640). After incubation, the beads were washed sequentially with a low salt buffer, high salt buffer, lithium chloride buffer, and TE buffer. DNA-protein complexes were eluted from the beads with 1% SDS TE buffer at 65°C for 15 min. Input and IP crosslinks were reversed by overnight incubation at 65°C. DNA was treated with proteinase K and RNase A before purification with the QIAquick PCR Purification kit.
NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NextSeq 500
Description input
Data processing Trim_galore
Genome_build: mm10
Supplementary_files_format_and_content: bigWig normalized read density
Submission date Aug 29, 2016
Last update date May 15, 2019
Contact name Bruce Horwitz
Organization name Brigham and Women's Hospital
Department Pathology
Street address 77 Ave Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL19057
Series (2)
GSE86169 Inhibition of inflammatory gene transcription by IL-10 is associated with rapid suppression of LPS-induced enhancer activation [stat3_ChIP]
GSE86170 Inhibition of inflammatory gene transcription by IL-10 is associated with rapid suppression of LPS-induced enhancer activation
BioSample SAMN05713628
SRA SRX2060974

Supplementary file Size Download File type/resource 135.7 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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