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Sample GSM2296663 Query DataSets for GSM2296663
Status Public on Feb 09, 2017
Title 195minLPS+30min-a10 Rep1
Sample type SRA
 
Source name Bone Marrow Derived Macrophages
Organism Mus musculus
Characteristics genotype: IL10-/-RAG2-/-
strain: 129S6/SvEvTac
cell type: Bone Marrow Derived Macrophage
Treatment protocol Untreated, LPS (1ng/mL) for 195min, LPS and IL-10 (1ng/mL) for 195min (c10), LPS for 195min and IL-10 for the last 30min of the stimulation (a10)
Growth protocol Bone marrow precursors were grown in M-CSF conditioned media containing FBS for 5 days prior to stimulation
Extracted molecule total RNA
Extraction protocol Cells were rinsed in PBS and lysed in TRIzol, RNA was purified with qiagen RNeasy Columns
NEB next ribomal depletion followed by NEB Directional RNA Library Prep Kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description 195min LPS +30min IL-10 (a10)
Data processing Trim_galore
Rbowtie
QuasR qCount
DESeq
Genome_build: mm10
Supplementary_files_format_and_content: csv of normalized expression values
 
Submission date Aug 29, 2016
Last update date May 15, 2019
Contact name Bruce Horwitz
E-mail(s) bhorwitz@rics.bwh.harvard.edu
Organization name Brigham and Women's Hospital
Department Pathology
Street address 77 Ave Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL19057
Series (2)
GSE86167 Inhibition of inflammatory gene transcription by IL-10 is associated with rapid suppression of LPS-induced enhancer activation [RNA-Seq]
GSE86170 Inhibition of inflammatory gene transcription by IL-10 is associated with rapid suppression of LPS-induced enhancer activation
Relations
BioSample SAMN05713582
SRA SRX2060948

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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