|
| Status |
Public on Sep 19, 2007 |
| Title |
SHED247 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
T0 sWAT
|
| Organism |
Homo sapiens |
| Characteristics |
Subcutaneous WAT of obese subjects before bariatric surgery
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from scWAT was prepared using the RNeasy RNA Mini Kit (Qiagen, Courtaboeuf, France).
|
| Label |
Cy3
|
| Label protocol |
One microgram of total RNA from each sample preparation was amplified using the MessageAmp RNA kit (Ambion, Austin, TX), and 3 µg aRNA was Cy-dye labeled using the CyScribe first-strand cDNA labeling kit (Amersham Biosciences, Orsay, France) (available at http://cmgm.stanford.edu/pbrown/protocols/index).
|
| |
|
| Channel 2 |
| Source name |
T3M sWAT
|
| Organism |
Homo sapiens |
| Characteristics |
Subcutaneous WAT of obese subjects 3 months after bariatric surgery
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from scWAT was prepared using the RNeasy RNA Mini Kit (Qiagen, Courtaboeuf, France).
|
| Label |
Cy5
|
| Label protocol |
One microgram of total RNA from each sample preparation was amplified using the MessageAmp RNA kit (Ambion, Austin, TX), and 3 µg aRNA was Cy-dye labeled using the CyScribe first-strand cDNA labeling kit (Amersham Biosciences, Orsay, France) (available at http://cmgm.stanford.edu/pbrown/protocols/index).
|
| |
|
| |
| Hybridization protocol |
The labeled cDNA mixtures were hybridized according to the protocol described at http://cmgm.stanford.edu/pbrown/protocols/index
|
| Scan protocol |
The arrays were immediately scanned using a GenePix 4000A confocal Scanner (Axon Instruments). Images were analyzed using GenePix pro 3 software.
|
| Description |
Seventeen Caucasian morbidly obese women undergoing laparoscopic Roux-en-Y bypass were enrolled at Hôtel Dieu Hospital, Paris, France. To study the effects of weight loss on scWAT gene expression, we processed 10 high-quality RNAs from scWAT biopsies of the 17 collected before/after bypass by cDNA microarray technique.
|
| Data processing |
After scanning the arrays, the images were analyzed and ratio intensities were filtered and normalized using Stanford Microarray Database Online resources (http://genome-www5.stanford.edu/).The VALUE field represents the log(base2) of the print-tip loess normalized R/G ratio (mean).
|
| |
|
| Submission date |
Sep 11, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Corneliu Henegar |
| E-mail(s) |
corneliu@henegar.info, flavien.jacques@upmc.fr
|
| Organization name |
Institut national de la santé et de la recherche médicale
|
| Department |
U872 Eq7
|
| Lab |
Eq7 Nutriomique
|
| Street address |
15 Rue de l'Ecole de Medecine
|
| City |
Paris |
| State/province |
Ile de France |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL3942 |
| Series (2) |
| GSE9007 |
Gene expression profiling of human adipose tissue of obese subjects in relation to bariatric surgery |
| GSE9157 |
Expression profiling of human adipose tissue in obese and lean subjects and in various clinical conditions |
|
