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Sample GSM2281912 Query DataSets for GSM2281912
Status Public on Aug 17, 2016
Title Normal Endometrium Sample - Control 03
Sample type genomic
 
Channel 1
Source name Eutopic endometrial tissue - control
Organism Homo sapiens
Characteristics Sex: female
Extracted molecule genomic DNA
Extraction protocol genomic DNA extracted and purification followed manufacturer's instructions (MasterPure™ DNA Purification Kit- Epicentre, Illumina).
Label Cy5
Label protocol 1ug of gDNA for each sample labeled (test and control) were required. The references were labelled with Cy3-dUTP and endometrium DNA samples were labelled with Cy5-dUTP. The SureTag Complete DNA Labeling kit was used following manufacturer's instructions.
 
Channel 2
Source name Commercial DNA Control Promega
Organism Homo sapiens
Characteristics Sex: female
Extracted molecule genomic DNA
Extraction protocol genomic DNA extracted and purification followed manufacturer's instructions (MasterPure™ DNA Purification Kit- Epicentre, Illumina).
Label Cy3
Label protocol 1ug of gDNA for each sample labeled (test and control) were required. The references were labelled with Cy3-dUTP and endometrium DNA samples were labelled with Cy5-dUTP. The SureTag Complete DNA Labeling kit was used following manufacturer's instructions.
 
 
Hybridization protocol The hybridization was performed according Agilent's protocol for a-CGH 2x400K. After hybridization, slides were washed sequential
Scan protocol Arrays were scanned using an Agilent microarray scanner (Agilent Technologies, Inc), and processed using Agilent’s Feature Extraction software followed manufacturer's instructions.
Description Health woman without endometriosis diagnosis neither familial history of the disease.
Data processing Agilent Feature Extraction Software (Protocol: CGH_107_sep09; Template:021850_D_F_20111015) was used for background subtraction and normalization.
 
Submission date Aug 16, 2016
Last update date Aug 17, 2016
Contact name Flavia Gaona de Oliveira Gennaro
E-mail(s) flaviagaona@usp.br
Phone +5516991567254
Organization name USP
Department Genetics
Lab Human Molecular Cytogenetics
Street address Av Bandeirantes, 3900, Depto. Genética, Bloco C
City Ribeirão Preto
State/province São Paulo
ZIP/Postal code 14049900
Country Brazil
 
Platform ID GPL9777
Series (1)
GSE85701 Identification of rare copy number polymorphic gains at 3q12.2 and 19q13.2 identifies candidate genes for familial endometriosis

Data table header descriptions
ID_REF
VALUE Normalized log10 ratio (Cy5/Cy3) representing test/reference.

Data table
ID_REF VALUE
1 -1.27E-02
2 0.00E+00
3 0.00E+00
4 -6.75E-02
5 8.07E-02
6 -5.26E-02
7 -4.55E-02
8 -6.63E-03
9 -7.10E-02
10 8.85E-04
11 -8.47E-02
12 -2.30E-02
13 5.90E-02
14 -6.09E-02
15 5.76E-02
16 -4.96E-03
17 -6.84E-02
18 -2.52E-02
19 -4.59E-02
20 6.27E-02

Total number of rows: 420288

Table truncated, full table size 6694 Kbytes.




Supplementary file Size Download File type/resource
GSM2281912_US09473741_252185021320_S01_CGH_107_Sep09_1_1.txt.gz 120.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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