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Sample GSM2278684 Query DataSets for GSM2278684
Status Public on Oct 30, 2016
Title miRNAs from PC73 patient
Sample type RNA
 
Source name serum miRNA (pancreatic cancer)
Organism Homo sapiens
Characteristics gender: M
age: 75
ca19-9: >37
Treatment protocol Serum samples were collected in 10-mL BD serum tubes and centrifuged at 4°C for 20 minutes at 3000 rpm.
Extracted molecule total RNA
Extraction protocol Total RNA containing miRNA was extracted from the serum samples using the Serum miRNA purification kit (Genolution, Seoul, Korea) according to the manufacturer’s instructions, and the RNA was resuspended with 12 μL of RNase-free water and stored at -80°C until microarray or qRT-PCR
Label biotin
Label protocol RNA samples (130 ng) were labelled using the FlashTag™ Biotin RNA Labeling Kit (Genisphere, Hatfield, PA)
 
Hybridization protocol labelled RNA was heated to 99°C for 5 minutes and then to 45°C for 5 minutes. RNA-array hybridization was performed with agitation at 60 rotations per minute for 16 hours at 48°C on an Affymetrix® 450 Fluidics Station
Scan protocol The chips were washed and stained using a Genechip Fluidics Station 450 (Affymetrix). The chips were then scanned using an Affymetrix GCS 3000 scanner
Description serum miRNAs from PC patient
Data processing cel files were analysed and rma-normalized by using the Expression Console software
 
Submission date Aug 15, 2016
Last update date Oct 30, 2016
Contact name Jaehoon Lee
E-mail(s) jae.h.lee@lge.com
Phone 82-10-9879-9131
Organization name LG Electronics
Street address 38, Baumoe-ro
City Seoul
ZIP/Postal code 137-724
Country South Korea
 
Platform ID GPL19117
Series (1)
GSE85589 Expression data from pancreatic cancers patients and healthy controls

Data table header descriptions
ID_REF
VALUE log2-rma

Data table
ID_REF VALUE
MIMAT0000062_st 2.025340902
MIMAT0004481_st 0.927038047
MIMAT0010195_st 0.853779482
MIMAT0000063_st 2.991540638
MIMAT0004482_st 1.201662085
MIMAT0000064_st 2.785624374
MIMAT0026472_st 0.934102695
MIMAT0000065_st 1.828856447
MIMAT0004484_st 0.698359073
MIMAT0000066_st 0.899142049
MIMAT0004485_st 0.62725428
MIMAT0000067_st 0.748470035
MIMAT0004486_st 0.572437549
MIMAT0004487_st 0.611819687
MIMAT0000068_st 1.000651254
MIMAT0004488_st 0.788888755
MIMAT0000069_st 3.668732891
MIMAT0004489_st 1.266793389
MIMAT0000070_st 2.88622217
MIMAT0000071_st 0.49166979

Total number of rows: 2578

Table truncated, full table size 70 Kbytes.




Supplementary file Size Download File type/resource
GSM2278684_PC73.CEL.gz 670.4 Kb (ftp)(http) CEL
Processed data included within Sample table

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