GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM227866 Query DataSets for GSM227866
Status Public on Jul 03, 2008
Title Mouth_15 (Never Smoker)
Sample type RNA
Source name Buccal mucosa sample from Never Smoker
Organism Homo sapiens
Characteristics Never Smoker
Treatment protocol Buccal mucosa samples collected from cheek scrapings, stored in RNA later; Nasal epithelial samples collected by brushing inferior nasal turbinate, brushes stored in RNA later.
Growth protocol Cells collected from human samples.
Extracted molecule total RNA
Extraction protocol Trizol extraction of buccal mucosa samples performed based on manufacturer's protocol, with RNA from serial collections from same patient pooled to obtain minimum of 8ug for hybridization to HG-U133A arrays; Nasal epithelial RNA isolated via Qiagen RNeasy Mini Kits per manufacturer’s protocol, with enough RNA from one collection to hybridize to HG-U133A arrays.
Label biotin
Label protocol Eight micrograms of total RNA from bronchial epithelial cells were converted into double-stranded cDNA with the SuperScript II reverse transcriptase (Invitrogen) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, Boulder, CO). The ENZO Bioarray RNA transcript labeling kit (Affymetrix) was used for in vitro transcription of the purified double-stranded cDNA.
Hybridization protocol The biotin-labeled cRNA was purified by using the RNeasy kit (Qiagen, Valencia, CA) and fragmented into ~ 200 base pairs by alkaline treatment (200 mM Tris-acetate, pH 8.2/500 mM potassium acetate/150 mM magnesium acetate). Each verified cRNA sample was then hybridized overnight onto the Affymetrix HG-U133A array.
Scan protocol Confocal laser scanning (Agilent) performed to detect the streptavidin-labeled fluor.
Description Gene expression data from never smoker buccal mucosa sample
Data processing A single weighted mean expression level for each gene was derived using MICROARRAY SUITE 5.0 (MAS 5.0) software . The MAS 5.0 software also generated a detection P value [P(detection)] using a one-sided Wilcoxon sign-ranked test, which indicated whether the transcript was reliably detected. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Sep 07, 2007
Last update date Aug 14, 2011
Contact name Sriram Sridhar
Phone 6178771485
Organization name MedImmune
Department Translational Medicine
Street address 1 MedImmune Way
City Gaithersburg
State/province MD
ZIP/Postal code 20878
Country USA
Platform ID GPL96
Series (1)
GSE8987 Expression data from buccal and nasal epithelium of current and never smokers

Data table header descriptions
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)

Data table
AFFX-BioB-5_at 395.087 P 0.000445901
AFFX-BioB-M_at 591.532 P 9.4506e-05
AFFX-BioB-3_at 332.099 P 7.00668e-05
AFFX-BioC-5_at 1274.96 P 8.14279e-05
AFFX-BioC-3_at 1136.31 P 5.16732e-05
AFFX-BioDn-5_at 1456.94 P 4.42873e-05
AFFX-BioDn-3_at 6725.06 P 0.000195116
AFFX-CreX-5_at 13493.6 P 4.42873e-05
AFFX-CreX-3_at 18636.6 P 4.42873e-05
AFFX-DapX-5_at 12.2155 A 0.455413
AFFX-DapX-M_at 16.2357 A 0.544587
AFFX-DapX-3_at 4.01834 A 0.976071
AFFX-LysX-5_at 3.27448 A 0.852061
AFFX-LysX-M_at 6.26605 A 0.971543
AFFX-LysX-3_at 3.65081 A 0.712257
AFFX-PheX-5_at 6.25816 A 0.783476
AFFX-PheX-M_at 6.31701 A 0.876428
AFFX-PheX-3_at 65.7659 A 0.39692
AFFX-ThrX-5_at 16.1387 A 0.783476
AFFX-ThrX-M_at 23.108 A 0.455409

Total number of rows: 22283

Table truncated, full table size 652 Kbytes.

Supplementary file Size Download File type/resource
GSM227866.CEL.gz 3.1 Mb (ftp)(http) CEL
GSM227866.CHP.gz 5.5 Mb (ftp)(http) CHP
GSM227866.EXP.gz 503 b (ftp)(http) EXP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap