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Sample GSM2278634 Query DataSets for GSM2278634
Status Public on Oct 30, 2016
Title miRNAs from PC23 patient
Sample type RNA
Source name serum miRNA (pancreatic cancer)
Organism Homo sapiens
Characteristics gender: F
age: 76
ca19-9: >37
Treatment protocol Serum samples were collected in 10-mL BD serum tubes and centrifuged at 4°C for 20 minutes at 3000 rpm.
Extracted molecule total RNA
Extraction protocol Total RNA containing miRNA was extracted from the serum samples using the Serum miRNA purification kit (Genolution, Seoul, Korea) according to the manufacturer’s instructions, and the RNA was resuspended with 12 μL of RNase-free water and stored at -80°C until microarray or qRT-PCR
Label biotin
Label protocol RNA samples (130 ng) were labelled using the FlashTag™ Biotin RNA Labeling Kit (Genisphere, Hatfield, PA)
Hybridization protocol labelled RNA was heated to 99°C for 5 minutes and then to 45°C for 5 minutes. RNA-array hybridization was performed with agitation at 60 rotations per minute for 16 hours at 48°C on an Affymetrix® 450 Fluidics Station
Scan protocol The chips were washed and stained using a Genechip Fluidics Station 450 (Affymetrix). The chips were then scanned using an Affymetrix GCS 3000 scanner
Description serum miRNAs from PC patient
Data processing cel files were analysed and rma-normalized by using the Expression Console software
Submission date Aug 15, 2016
Last update date Oct 30, 2016
Contact name Jaehoon Lee
Phone 82-10-9879-9131
Organization name LG Electronics
Street address 38, Baumoe-ro
City Seoul
ZIP/Postal code 137-724
Country South Korea
Platform ID GPL19117
Series (1)
GSE85589 Expression data from pancreatic cancers patients and healthy controls

Data table header descriptions
VALUE log2-rma

Data table
MIMAT0000062_st 1.050355665
MIMAT0004481_st 0.679628631
MIMAT0010195_st 0.630374792
MIMAT0000063_st 5.743915447
MIMAT0004482_st 0.735284717
MIMAT0000064_st 4.008180673
MIMAT0026472_st 0.74487342
MIMAT0000065_st 1.290252709
MIMAT0004484_st 0.505111796
MIMAT0000066_st 0.878842091
MIMAT0004485_st 0.556506735
MIMAT0000067_st 0.818521734
MIMAT0004486_st 1.345664985
MIMAT0004487_st 0.335680259
MIMAT0000068_st 0.854066243
MIMAT0004488_st 0.550752667
MIMAT0000069_st 3.89522887
MIMAT0004489_st 0.747303351
MIMAT0000070_st 3.364141548
MIMAT0000071_st 0.989931228

Total number of rows: 2578

Table truncated, full table size 70 Kbytes.

Supplementary file Size Download File type/resource
GSM2278634_PC23.CEL.gz 655.6 Kb (ftp)(http) CEL
Processed data included within Sample table

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