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Sample GSM2257348 Query DataSets for GSM2257348
Status Public on Jan 28, 2017
Title Healthy human brain
Sample type SRA
Source name Total RNA from human brain
Organism Homo sapiens
Characteristics tissue: brain
diagnosis: normal brain
Extracted molecule total RNA
Extraction protocol Total RNA isolated using modified guanidine thiocyanate technique from healthy human and Alzheimer’s disease brain was procured from BioChain, USA. The purity of isolated RNA was examined using NanoDrop spectrophotometer (Thermo Scientific) by measuring absorbance at 260 nm and 280 nm. An aliquot of each the samples was also run on an Agilent RNA Bioanalyzer to check the RNA integrity number (RIN) and was found to be 8.1 and 8.5 in HB and AD sample respectively.
The RNA libraries were constructed for sequencing following Illumina TruSeq Small RNA library protocol outlined in “TruSeq Small RNA Sample Preparation Guide” (Part # 15004197; Rev. F; February 2014) and using TruSeq Small RNA sample preparation kit (Illumina, # RS-200-0012).
Library strategy ncRNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina NextSeq 500
Description small RNA
Data processing bcl2fastq software (Illumina) was used for converting image file to FASTq format.
Sequenced reads were trimmed for adaptor sequence using srna Work bench, and Quality check of the generated data was done using the FASTqc software.
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files of annotated piRNAs identified in each Sample. A text file contains the read counts for miRNAs.
Submission date Aug 01, 2016
Last update date May 15, 2019
Contact name Dr. Bibekanand Mallick
Organization name National Institute of Technology
Department Department of Life Science
Lab RNAi & Functional Genomics Lab.
Street address NIT Rourkela
City Rourkela
State/province Odisha
ZIP/Postal code 769008
Country India
Platform ID GPL18573
Series (1)
GSE85075 Next-Generation Sequencing identified piRNA profiles in healthy and Alzheimer’s-affected human brain
BioSample SAMN05505396
SRA SRX1995255

Supplementary file Size Download File type/resource
GSM2257348_miRNAs_Healthy_brain.txt.gz 9.3 Kb (ftp)(http) TXT
GSM2257348_piRNAs_Healthy_brain.txt.gz 10.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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