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Sample GSM2254428 Query DataSets for GSM2254428
Status Public on Jun 10, 2017
Title PDX-ET-743-2 [miRNA]
Sample type RNA
Source name PDX ET-743
Organism Homo sapiens
Characteristics tissue: CHC001
cell/tissue type: intrahepatic cholangiocarcinoma paired patient derived xenograft (ICC PDX)
treated with: ET-743 (0.15 mg/Kg weekly i.v., three administrations)
Treatment protocol For in vitro experiment: 24 hours at 5 nM of ET-743. In Vivo: 3 weeks of treatement with ET-743 at the dose of 0.15 mg/Kg/weekly i.v.
Growth protocol MT-CHC01 cells were cultured in KODMEM-F12 with glutammine, Hepes and P/S
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol and Absolute RNA miRNA kit (Agilent) following manufacturer's instructions
Label Cy3
Label protocol One hundred ng of total RNA were labeled using the miRNA Complete Labeling and Hyb Kit
Hybridization protocol labeled RNA were hybridized on Human miRNA Microarray Kit Release 16.0, 8x60K
Scan protocol Images were quantified using Agilent Feature Extraction Software (version 10.7).
Data processing raw data were then processed using the Bioconductor package Limma (Linear models for microarray analysis).
Submission date Jul 28, 2016
Last update date Jun 10, 2017
Contact name giovanna chiorino
Organization name Fondo Edo Tempia
Department Cancer Genomics
Street address via malta 3
City Biella
ZIP/Postal code 13900
Country Italy
Platform ID GPL15159
Series (2)
GSE84938 Gene and microRNA modulation upon ET-743 treatment in a human intrahepatic cholangiocarcinoma paired patient derived xenograft and cell line [miRNA expression]
GSE84939 Gene and microRNA modulation upon ET-743 treatment in a human intrahepatic cholangiocarcinoma paired patient derived xenograft and cell line

Data table header descriptions
VALUE quantile normalized log2 intensities

Data table
A_25_P00013722 6.782898762
A_25_P00015543 6.76400207
A_25_P00013773 6.768256615
A_25_P00015229 6.86031939
A_25_P00016018 6.771217581
A_25_P00011991 7.831563462
A_25_P00015101 6.74136453
A_25_P00010432 6.950016521
A_25_P00015836 7.173688301
A_25_P00010259 6.84492881
A_25_P00015285 6.76296986
A_25_P00015097 6.840221913
A_25_P00012376 10.37074311
A_25_P00012139 14.38467636
A_25_P00015493 6.774706561
A_25_P00012297 6.755919586
A_25_P00010683 9.038578751
A_25_P00015031 6.896411111
A_25_P00015444 6.855469972
A_25_P00012542 6.850175745

Total number of rows: 3523

Table truncated, full table size 92 Kbytes.

Supplementary file Size Download File type/resource
GSM2254428_US22502614_253118112455_S01_miRNA_107_Sep09_2_2.txt.gz 7.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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