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Sample GSM2254427 Query DataSets for GSM2254427
Status Public on Jun 10, 2017
Title PDX-ET-743-1 [miRNA]
Sample type RNA
Source name PDX ET-743
Organism Homo sapiens
Characteristics tissue: CHC001
cell/tissue type: intrahepatic cholangiocarcinoma paired patient derived xenograft (ICC PDX)
treated with: ET-743 (0.15 mg/Kg weekly i.v., three administrations)
Treatment protocol For in vitro experiment: 24 hours at 5 nM of ET-743. In Vivo: 3 weeks of treatement with ET-743 at the dose of 0.15 mg/Kg/weekly i.v.
Growth protocol MT-CHC01 cells were cultured in KODMEM-F12 with glutammine, Hepes and P/S
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol and Absolute RNA miRNA kit (Agilent) following manufacturer's instructions
Label Cy3
Label protocol One hundred ng of total RNA were labeled using the miRNA Complete Labeling and Hyb Kit
Hybridization protocol labeled RNA were hybridized on Human miRNA Microarray Kit Release 16.0, 8x60K
Scan protocol Images were quantified using Agilent Feature Extraction Software (version 10.7).
Data processing raw data were then processed using the Bioconductor package Limma (Linear models for microarray analysis).
Submission date Jul 28, 2016
Last update date Jun 10, 2017
Contact name giovanna chiorino
Organization name Fondo Edo Tempia
Department Cancer Genomics
Street address via malta 3
City Biella
ZIP/Postal code 13900
Country Italy
Platform ID GPL15159
Series (2)
GSE84938 Gene and microRNA modulation upon ET-743 treatment in a human intrahepatic cholangiocarcinoma paired patient derived xenograft and cell line [miRNA expression]
GSE84939 Gene and microRNA modulation upon ET-743 treatment in a human intrahepatic cholangiocarcinoma paired patient derived xenograft and cell line

Data table header descriptions
VALUE quantile normalized log2 intensities

Data table
A_25_P00013722 6.783247445
A_25_P00015543 6.694697504
A_25_P00013773 6.759456622
A_25_P00015229 6.791890665
A_25_P00016018 6.779613395
A_25_P00011991 7.521333244
A_25_P00015101 6.797912254
A_25_P00010432 6.994420694
A_25_P00015836 7.157549818
A_25_P00010259 6.751919161
A_25_P00015285 6.79399866
A_25_P00015097 6.724325514
A_25_P00012376 9.895987445
A_25_P00012139 13.95774338
A_25_P00015493 6.721541677
A_25_P00012297 6.642125
A_25_P00010683 9.280186294
A_25_P00015031 6.795271215
A_25_P00015444 6.868768492
A_25_P00012542 6.804691192

Total number of rows: 3523

Table truncated, full table size 92 Kbytes.

Supplementary file Size Download File type/resource
GSM2254427_US22502614_253118112455_S01_miRNA_107_Sep09_1_2.txt.gz 7.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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