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Sample GSM2254423 Query DataSets for GSM2254423
Status Public on Jun 10, 2017
Title MT-CHC01-ET-743-1 [miRNA]
Sample type RNA
 
Source name MT-CHC01 ET-743
Organism Homo sapiens
Characteristics cell line: MT-CHC01
cell/tissue type: intrahepatic cholangiocarcinoma cell line
treated with: 5 nM ET-743 for 24 hrs
Treatment protocol For in vitro experiment: 24 hours at 5 nM of ET-743. In Vivo: 3 weeks of treatement with ET-743 at the dose of 0.15 mg/Kg/weekly i.v.
Growth protocol MT-CHC01 cells were cultured in KODMEM-F12 with glutammine, Hepes and P/S
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol and Absolute RNA miRNA kit (Agilent) following manufacturer's instructions
Label Cy3
Label protocol One hundred ng of total RNA were labeled using the miRNA Complete Labeling and Hyb Kit
 
Hybridization protocol labeled RNA were hybridized on Human miRNA Microarray Kit Release 16.0, 8x60K
Scan protocol Images were quantified using Agilent Feature Extraction Software (version 10.7).
Data processing raw data were then processed using the Bioconductor package Limma (Linear models for microarray analysis).
 
Submission date Jul 28, 2016
Last update date Jun 10, 2017
Contact name giovanna chiorino
E-mail(s) giovanna.chiorino@gmail.com
Organization name Fondo Edo Tempia
Department Cancer Genomics
Street address via malta 3
City Biella
ZIP/Postal code 13900
Country Italy
 
Platform ID GPL15159
Series (2)
GSE84938 Gene and microRNA modulation upon ET-743 treatment in a human intrahepatic cholangiocarcinoma paired patient derived xenograft and cell line [miRNA expression]
GSE84939 Gene and microRNA modulation upon ET-743 treatment in a human intrahepatic cholangiocarcinoma paired patient derived xenograft and cell line

Data table header descriptions
ID_REF
VALUE quantile normalized log2 intensities

Data table
ID_REF VALUE
A_25_P00013722 6.135190905
A_25_P00015543 6.169993333
A_25_P00013773 5.988107667
A_25_P00015229 6.310432719
A_25_P00016018 6.259428392
A_25_P00011991 7.122359873
A_25_P00015101 6.043569631
A_25_P00010432 6.135330679
A_25_P00015836 6.107388917
A_25_P00010259 6.170610151
A_25_P00015285 6.181119483
A_25_P00015097 6.295840311
A_25_P00012376 7.439094221
A_25_P00012139 11.26806976
A_25_P00015493 6.087357667
A_25_P00012297 6.028912701
A_25_P00010683 6.784754158
A_25_P00015031 6.251815531
A_25_P00015444 6.291670783
A_25_P00012542 6.233497759

Total number of rows: 3523

Table truncated, full table size 92 Kbytes.




Supplementary file Size Download File type/resource
GSM2254423_US22502614_253118114366_S02_miRNA_107_Sep09_2_1.txt.gz 7.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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