NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2254205 Query DataSets for GSM2254205
Status Public on Jun 10, 2017
Title CHC001 PDX ET-743 - replicate 2
Sample type RNA
 
Source name CHC001 PDX ET-743 - replicate 2
Organism Homo sapiens
Characteristics cell line: CHC001 PDX ET-743 (0.15 mg/Kg weekly i.v., three administrations)
treatment: ET-743 at the dose of 0.15 mg/Kg/weekly for three weeks
Treatment protocol For in vitro experiment: 24 hours at 5 nM of ET-743. In Vivo: 3 weeks of treatement with ET-743 at the dose of 0.15 mg/Kg/weekly i.v.
Growth protocol MT-CHC01 cells were cultured in KODMEM-F12 with 10% fbs, glutammine, Hepes and P/S
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol AND Absolute RNA miRNA kit (Agilent) following manufacturer's instructions
Label Cy3
Label protocol 100 ng of total RNA were amplified and labeled using Low Input Quick Amp Labeling Kit, one-color kit (Agilent Technologies);
 
Hybridization protocol Six hundred ng of labeled RNA were hybridized on SurePrint G3 Human Gene Expression 8x60K v2 glass arrays;
Scan protocol Images were quantified using Agilent Feature Extraction Software (version 10.7).
Data processing raw data were then processed using the Bioconductor package Limma (Linear models for microarray analysis).
 
Submission date Jul 27, 2016
Last update date Jun 10, 2017
Contact name giovanna chiorino
E-mail(s) giovanna.chiorino@gmail.com
Organization name Fondo Edo Tempia
Department Cancer Genomics
Street address via malta 3
City Biella
ZIP/Postal code 13900
Country Italy
 
Platform ID GPL17077
Series (2)
GSE84918 Gene and microRNA modulation upon ET-743 treatment in a human intrahepatic cholangiocarcinoma paired patient derived xenograft and cell line [gene expression]
GSE84939 Gene and microRNA modulation upon ET-743 treatment in a human intrahepatic cholangiocarcinoma paired patient derived xenograft and cell line

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
A_23_P117082 12.26002659
A_33_P3246448 7.00783172
A_33_P3318220 6.387339718
A_33_P3236322 6.725626654
A_33_P3319925 7.923761629
A_21_P0000509 15.1362471
A_21_P0000744 9.864395583
A_24_P215804 6.610487569
A_23_P110167 13.40434494
A_33_P3211513 11.18064476
A_23_P103349 6.841418068
A_32_P61480 6.860506886
A_33_P3788124 6.833914538
A_33_P3414202 10.63588468
A_33_P3316686 10.76025956
A_33_P3300975 9.677663406
A_33_P3263061 12.46662655
A_33_P3261373 6.675441785
A_24_P278460 10.00804722
A_21_P0013109 6.663015861

Total number of rows: 50599

Table truncated, full table size 1255 Kbytes.




Supplementary file Size Download File type/resource
GSM2254205_US22502614_253949410796_S01_GE1_107_Sep09_2_NO_SPIKEIN_2_2.txt.gz 12.5 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap