self-fertile and virulent on host plants: Oryza sativa L. cv. Dongjin cultivar: Oryza sativa L. cv. Dongjin
Treatment protocol
The calli were induced from seeds on 2N6 medium at 37°C for 4 weeks. The regenerating calli were induced from calli for 2 weeks on a MS16 medium and collected when turned green. The seeds were germinated on water-soaked filter paper for 4 days in a dark chamber. Leaves and roots were harvested separately 14 days after sowing in soil. A flower sample was harvested at exact stages following recommendations from previous research. The flowers before pollination were prepared just prior to the opening of the spikelet, and the flowers after pollination were collected followed by anthesis, respectively. The flower before pollination (FBP) was collected prior to the opening of the spikelet, and its pistil length was in the range of 0.5 to 1.5 mm. The flower after pollination (FAP) was collected after the anthesis and maximum opening of the florets.
Growth protocol
All seven tissues in this study were prepared from Oryza sativa L. cv. Dongjin.
Extracted molecule
total RNA
Extraction protocol
Total RNAs were extracted using TriReagent (Molecular Research Center, Inc.).
Label
Cy3
Label protocol
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
Hybridization protocol
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
Scan protocol
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
Description
Roots were harvested separately 14 days after sowing in soil.
Data processing
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
