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Sample GSM2242448 Query DataSets for GSM2242448
Status Public on Jul 31, 2017
Title H7
Sample type RNA
 
Source name Striatum
Organism Rattus norvegicus
Characteristics treatment group: Acute
tissue: brain
tissue compartment: Striatum
Sex: male
age: 80-90 days old
strain: Long Evans
Treatment protocol Rats were randomly assigned to 1 of 3 treatment groups (saline, acute, repeated/chronic). Saline rats received one daily saline injection (i.p.) on 5 days, acute rats received one daily saline injection on 4 days and a final MDPV injection on a 5th day, repeated MDPV rats received one daily MDPV injection on 5 days. MDPV dose was 1 mg/kg. Twenty four hours after the final injection, rats were euthanized, brains removed, flash frozen and stored at -80C until day of assay. For assay, left dorsal striatum was manually dissected to process in the following steps.
Extracted molecule total RNA
Extraction protocol Trizol protocol for total RNA following manufacturer's protocol
Label Cy3
Label protocol Agilent one color protocol for Cy3 following manufacturer's protocol
 
Hybridization protocol Agilent Technologies One-Color Microarray-Based Gene Expression Analysis Protocol was used. There was 825 ng of Cy3-labeled cRNA used.
Scan protocol Microarrays were kept in the dark until scanned at 5 um, channel G, Agilent HD (61x21.6mm) using the Agilent DNA Microarray Scanner with SureScan High ResolutionTechnology.
Data processing Agilent Feature Extraction Software (v10.7) was used. Quantile normalized.
 
Submission date Jul 19, 2016
Last update date Nov 13, 2022
Contact name Christopher Martyniuk
E-mail(s) cmartyn@ufl.edu
Phone 3523173905
Organization name University of Florida
Street address 8891 SW 79th Avenue
City Gainesville
State/province FL
ZIP/Postal code 32608
Country USA
 
Platform ID GPL22145
Series (1)
GSE84596 Effect of chronic MDPV on striatal gene expression

Data table header descriptions
ID_REF
VALUE Quantile normalized signal

Data table
ID_REF VALUE
56849 7.580797687
2965 12.637421
5724 12.62758335
7867 12.59282699
10987 12.59684422
16462 12.5920762
41221 12.73841185
43748 12.69479653
47538 12.58158655
50507 12.65182158
55655 12.6963382
40496 9.773892815
14745 10.54544165
57941 6.596108657
56714 10.64025169
57489 10.19716579
60230 5.253642411
36932 11.98347097
838 13.25528462
17708 13.36808111

Total number of rows: 58717

Table truncated, full table size 1015 Kbytes.




Supplementary file Size Download File type/resource
GSM2242448_US83800208_257403610204_S01_GE1_v5_10_Apr08_1_4.txt.gz 12.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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