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Sample GSM2242447 Query DataSets for GSM2242447
Status Public on Jul 31, 2017
Title D0
Sample type RNA
 
Source name Striatum
Organism Rattus norvegicus
Characteristics treatment group: Saline
tissue: brain
tissue compartment: Striatum
Sex: male
age: 80-90 days old
strain: Long Evans
Treatment protocol Rats were randomly assigned to 1 of 3 treatment groups (saline, acute, repeated/chronic). Saline rats received one daily saline injection (i.p.) on 5 days, acute rats received one daily saline injection on 4 days and a final MDPV injection on a 5th day, repeated MDPV rats received one daily MDPV injection on 5 days. MDPV dose was 1 mg/kg. Twenty four hours after the final injection, rats were euthanized, brains removed, flash frozen and stored at -80C until day of assay. For assay, left dorsal striatum was manually dissected to process in the following steps.
Extracted molecule total RNA
Extraction protocol Trizol protocol for total RNA following manufacturer's protocol
Label Cy3
Label protocol Agilent one color protocol for Cy3 following manufacturer's protocol
 
Hybridization protocol Agilent Technologies One-Color Microarray-Based Gene Expression Analysis Protocol was used. There was 825 ng of Cy3-labeled cRNA used.
Scan protocol Microarrays were kept in the dark until scanned at 5 um, channel G, Agilent HD (61x21.6mm) using the Agilent DNA Microarray Scanner with SureScan High ResolutionTechnology.
Data processing Agilent Feature Extraction Software (v10.7) was used. Quantile normalized.
 
Submission date Jul 19, 2016
Last update date Nov 13, 2022
Contact name Christopher Martyniuk
E-mail(s) cmartyn@ufl.edu
Phone 3523173905
Organization name University of Florida
Street address 8891 SW 79th Avenue
City Gainesville
State/province FL
ZIP/Postal code 32608
Country USA
 
Platform ID GPL22145
Series (1)
GSE84596 Effect of chronic MDPV on striatal gene expression

Data table header descriptions
ID_REF
VALUE Quantile normalized signal

Data table
ID_REF VALUE
56849 7.59459284
2965 12.76675099
5724 12.7613088
7867 12.71394188
10987 12.6963382
16462 12.77280197
41221 12.88048088
43748 12.87981368
47538 12.77521429
50507 12.81460123
55655 12.85146696
40496 9.846859375
14745 10.46872779
57941 6.174621173
56714 10.60542909
57489 10.0079313
60230 5.420285111
36932 11.66776882
838 13.29983911
17708 13.20167044

Total number of rows: 58717

Table truncated, full table size 1015 Kbytes.




Supplementary file Size Download File type/resource
GSM2242447_US83800208_257403610204_S01_GE1_v5_10_Apr08_1_3.txt.gz 12.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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