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Sample GSM2242445 Query DataSets for GSM2242445
Status Public on Jul 31, 2017
Title H3
Sample type RNA
 
Source name Striatum
Organism Rattus norvegicus
Characteristics treatment group: Saline
tissue: brain
tissue compartment: Striatum
Sex: male
age: 80-90 days old
strain: Long Evans
Treatment protocol Rats were randomly assigned to 1 of 3 treatment groups (saline, acute, repeated/chronic). Saline rats received one daily saline injection (i.p.) on 5 days, acute rats received one daily saline injection on 4 days and a final MDPV injection on a 5th day, repeated MDPV rats received one daily MDPV injection on 5 days. MDPV dose was 1 mg/kg. Twenty four hours after the final injection, rats were euthanized, brains removed, flash frozen and stored at -80C until day of assay. For assay, left dorsal striatum was manually dissected to process in the following steps.
Extracted molecule total RNA
Extraction protocol Trizol protocol for total RNA following manufacturer's protocol
Label Cy3
Label protocol Agilent one color protocol for Cy3 following manufacturer's protocol
 
Hybridization protocol Agilent Technologies One-Color Microarray-Based Gene Expression Analysis Protocol was used. There was 825 ng of Cy3-labeled cRNA used.
Scan protocol Microarrays were kept in the dark until scanned at 5 um, channel G, Agilent HD (61x21.6mm) using the Agilent DNA Microarray Scanner with SureScan High ResolutionTechnology.
Data processing Agilent Feature Extraction Software (v10.7) was used. Quantile normalized.
 
Submission date Jul 19, 2016
Last update date Nov 13, 2022
Contact name Christopher Martyniuk
E-mail(s) cmartyn@ufl.edu
Phone 3523173905
Organization name University of Florida
Street address 8891 SW 79th Avenue
City Gainesville
State/province FL
ZIP/Postal code 32608
Country USA
 
Platform ID GPL22145
Series (1)
GSE84596 Effect of chronic MDPV on striatal gene expression

Data table header descriptions
ID_REF
VALUE Quantile normalized signal

Data table
ID_REF VALUE
56849 7.934810031
2965 12.91303653
5724 12.86723457
7867 12.88401316
10987 12.96964707
16462 13.09168957
41221 13.11183652
43748 12.98390054
47538 12.93309184
50507 12.92782188
55655 12.99446088
40496 9.674496126
14745 10.50709345
57941 6.694912574
56714 10.18170481
57489 9.826945779
60230 5.768134539
36932 11.59255301
838 13.30636196
17708 13.29634098

Total number of rows: 58717

Table truncated, full table size 1015 Kbytes.




Supplementary file Size Download File type/resource
GSM2242445_US83800208_257403610204_S01_GE1_v5_10_Apr08_1_1.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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