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Sample GSM2242440 Query DataSets for GSM2242440
Status Public on Jul 31, 2017
Title E0
Sample type RNA
 
Source name Striatum
Organism Rattus norvegicus
Characteristics treatment group: Acute
tissue: brain
tissue compartment: Striatum
Sex: male
age: 80-90 days old
strain: Long Evans
Treatment protocol Rats were randomly assigned to 1 of 3 treatment groups (saline, acute, repeated/chronic). Saline rats received one daily saline injection (i.p.) on 5 days, acute rats received one daily saline injection on 4 days and a final MDPV injection on a 5th day, repeated MDPV rats received one daily MDPV injection on 5 days. MDPV dose was 1 mg/kg. Twenty four hours after the final injection, rats were euthanized, brains removed, flash frozen and stored at -80C until day of assay. For assay, left dorsal striatum was manually dissected to process in the following steps.
Extracted molecule total RNA
Extraction protocol Trizol protocol for total RNA following manufacturer's protocol
Label Cy3
Label protocol Agilent one color protocol for Cy3 following manufacturer's protocol
 
Hybridization protocol Agilent Technologies One-Color Microarray-Based Gene Expression Analysis Protocol was used. There was 825 ng of Cy3-labeled cRNA used.
Scan protocol Microarrays were kept in the dark until scanned at 5 um, channel G, Agilent HD (61x21.6mm) using the Agilent DNA Microarray Scanner with SureScan High ResolutionTechnology.
Data processing Agilent Feature Extraction Software (v10.7) was used. Quantile normalized.
 
Submission date Jul 19, 2016
Last update date Nov 13, 2022
Contact name Christopher Martyniuk
E-mail(s) cmartyn@ufl.edu
Phone 3523173905
Organization name University of Florida
Street address 8891 SW 79th Avenue
City Gainesville
State/province FL
ZIP/Postal code 32608
Country USA
 
Platform ID GPL22145
Series (1)
GSE84596 Effect of chronic MDPV on striatal gene expression

Data table header descriptions
ID_REF
VALUE Quantile normalized signal

Data table
ID_REF VALUE
56849 7.899245216
2965 12.86804501
5724 12.78245232
7867 12.79678296
10987 12.77635296
16462 12.8545158
41221 12.86749544
43748 12.86574647
47538 12.82225557
50507 12.79483476
55655 12.90137309
40496 9.898353639
14745 10.61330606
57941 7.12039648
56714 10.88522435
57489 9.744467874
60230 5.934285215
36932 12.08757935
838 13.55893225
17708 13.59160534

Total number of rows: 58717

Table truncated, full table size 1015 Kbytes.




Supplementary file Size Download File type/resource
GSM2242440_US83800208_257403610203_S01_GE1_v5_10_Apr08_1_4.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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