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Sample GSM2242437 Query DataSets for GSM2242437
Status Public on Jul 31, 2017
Title H10
Sample type RNA
 
Source name Striatum
Organism Rattus norvegicus
Characteristics treatment group: Chronic
tissue: brain
tissue compartment: Striatum
Sex: male
age: 80-90 days old
strain: Long Evans
Treatment protocol Rats were randomly assigned to 1 of 3 treatment groups (saline, acute, repeated/chronic). Saline rats received one daily saline injection (i.p.) on 5 days, acute rats received one daily saline injection on 4 days and a final MDPV injection on a 5th day, repeated MDPV rats received one daily MDPV injection on 5 days. MDPV dose was 1 mg/kg. Twenty four hours after the final injection, rats were euthanized, brains removed, flash frozen and stored at -80C until day of assay. For assay, left dorsal striatum was manually dissected to process in the following steps.
Extracted molecule total RNA
Extraction protocol Trizol protocol for total RNA following manufacturer's protocol
Label Cy3
Label protocol Agilent one color protocol for Cy3 following manufacturer's protocol
 
Hybridization protocol Agilent Technologies One-Color Microarray-Based Gene Expression Analysis Protocol was used. There was 825 ng of Cy3-labeled cRNA used.
Scan protocol Microarrays were kept in the dark until scanned at 5 um, channel G, Agilent HD (61x21.6mm) using the Agilent DNA Microarray Scanner with SureScan High ResolutionTechnology.
Data processing Agilent Feature Extraction Software (v10.7) was used. Quantile normalized.
 
Submission date Jul 19, 2016
Last update date Nov 13, 2022
Contact name Christopher Martyniuk
E-mail(s) cmartyn@ufl.edu
Phone 3523173905
Organization name University of Florida
Street address 8891 SW 79th Avenue
City Gainesville
State/province FL
ZIP/Postal code 32608
Country USA
 
Platform ID GPL22145
Series (1)
GSE84596 Effect of chronic MDPV on striatal gene expression

Data table header descriptions
ID_REF
VALUE Quantile normalized signal

Data table
ID_REF VALUE
56849 7.236092657
2965 12.84754591
5724 12.84299734
7867 12.81460123
10987 12.75286051
16462 12.90416925
41221 12.93685348
43748 12.85735864
47538 12.83292228
50507 12.75248894
55655 12.94931839
40496 9.648073751
14745 10.38524407
57941 6.329477557
56714 10.62369476
57489 10.54846991
60230 5.952839812
36932 11.74061628
838 13.26978984
17708 13.27498549

Total number of rows: 58717

Table truncated, full table size 1015 Kbytes.




Supplementary file Size Download File type/resource
GSM2242437_US83800208_257403610203_S01_GE1_v5_10_Apr08_1_1.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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