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Sample GSM2233141 Query DataSets for GSM2233141
Status Public on Jul 15, 2016
Title High2, biological rep2
Sample type RNA
Source name High concentration group, 600μM
Organism Homo sapiens
Characteristics cell line: HepG2
tissue: human hepatocellular carcinoma
Treatment protocol HepG2 cells were seeded in 10 cm dishes and cultured as usual until they reached 80% confluence. The cells were treated with H2O2 (0, 30 and 600 μM) for 24 h and each concentration was repeated in 3 dishes.
Growth protocol HepG2 cells were maintained as a monolayer culture in DMEM supplemented with 10% fetal bovine serum, 100 IU/ml penicillin and 100 μg/ml streptomycin. HepG2 cells were cultured in a humidified incubator with 5% CO2 at 37°C.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Per RNA sample, 1 ug were used as input into the procedure as recommended by protocol (Genisphere FlashTagTM Biotin RNA Labeling for Affymetrix GeneChip miRNA arrays).
Hybridization protocol Labeled miRNA was hybridized to the array for 16 hours at 48 “C and 60 rpm as described in the protocol.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G
Description miRNAs expression data from HepG2 cells treated with 600μM H2O2 for 24 hours
Data processing The data were analyzed with Robust Multi-chip Average (RMA) algorithm as normalization method.
Submission date Jul 14, 2016
Last update date Jul 15, 2016
Contact name Yuan LUO
Organization name Air Force General Hospital, PLA, P. R. China
Lab Aviation Medicine Research Laboratory
Street address No. 30 Fucheng Road, Haidian District
City Beijing
State/province Beijing
ZIP/Postal code 100142
Country China
Platform ID GPL19117
Series (1)
GSE84406 miRNAs array analysis of HepG2 cells under oxidative stress

Data table header descriptions
VALUE RMA normalized log2 (expression values)

Data table
14q0_st 1.137566 A
14qI-1_st 0.785639 A
14qI-1_x_st 1.011196 A
14qI-2_st 0.577875 A
14qI-3_x_st 1.020744 A
14qI-4_st 1.230334 A
14qI-4_x_st 1.064096 A
14qI-5_st 0.902557 A
14qI-6_st 0.752563 A
14qI-7_st 1.310278 A
14qI-8_st 0.902557 A
14qI-8_x_st 0.902557 A
14qI-9_x_st 1.304225 A
14qII-10_st 1.416363 P
14qII-11_st 0.902557 A
14qII-12_st 0.737406 A
14qII-12_x_st 1.061817 A
14qII-13_st 1.241244 A
14qII-14_st 1.039481 A
14qII-14_x_st 1.020744 A

Total number of rows: 6658

Table truncated, full table size 173 Kbytes.

Supplementary file Size Download File type/resource
GSM2233141_G2-600-2_miRNA-4_0_.CEL.gz 763.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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