|
| Status |
Public on Jun 26, 2017 |
| Title |
SKNO-1 H3K27me3 ASXL2 SH2 |
| Sample type |
SRA |
| |
|
| Source name |
SKNO1 cells
|
| Organism |
Homo sapiens |
| Characteristics |
fusion protein: AML-ETO
cell line: SKNO1
cell type: AML cell line
chip antibody: H3K27me3 antibody (Millipore 07-449)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
Libraries were prepared according to Illumina's instructions
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Low quality (< 15) bases were trimmed using cutadapt
Reads were aligned to genome assembly hg19 using Bowtie2 with default paramters
Duplicate reads were removed using Picard MarkDuplicates
The coverage data was converted to bed format using a custom script, and then to BigWig format
Genome_build: hg19
Supplementary_files_format_and_content: BigWig format
|
| |
|
| Submission date |
Jul 13, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Omar Abdel-Wahab |
| E-mail(s) |
abdelwao@mskcc.org
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Medicine
|
| Lab |
Omar Abdel-Wahab Lab
|
| Street address |
1275 York Ave.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (2) |
| GSE84362 |
ChIP-seq of H3K27me3 in SKNO1 cells after knockdown of ASXL2 |
| GSE84365 |
RNA-seq and ChIP-seq data of ASXL1/2 in Hematopoiesis |
|
| Relations |
| BioSample |
SAMN05388888 |
| SRA |
SRX1942896 |
