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Sample GSM2227792 Query DataSets for GSM2227792
Status Public on Jan 01, 2017
Title WT - 21 mon, biological rep3
Sample type RNA
Source name WT - 21 mo
Organism Mus musculus
Characteristics background strain: C57Bl/6J
genotype/variation: Wild type
tissue: Ventricular
Treatment protocol The hearts were collected and snap frozen in liquid Nitrogen.
Growth protocol The mice were collected at 4 or 21 months of age at ZT 6 ( 6 hours into the light phase)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the miRNeasy method according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng total RNA
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Data processing The data were analyzed with GeneSpring GX Software v. 11.5 using default Affymetrix settings.
Submission date Jul 07, 2016
Last update date Jan 01, 2017
Contact name Faisal James Alibhai
Organization name University of Guelph
Department Biomedical Sciences
Lab Martino Lab
Street address 50 Stone Rd East
City Guelph
State/province ON
ZIP/Postal code N1G2W1
Country Canada
Platform ID GPL19117
Series (1)
GSE84151 miRNA Expression data from murine hearts of transgenic Clock delta 19/delta 19 mice (Clock mice) and wild type (WT) mice at 4 and 21 months of age

Data table header descriptions
VALUE Normalized signal

Data table
MIMAT0000121_st 0.1413641
MIMAT0004519_st 0.39724112
MIMAT0000122_st 0.07438183
MIMAT0004520_st -0.10431218
MIMAT0016979_st -0.24215204
MIMAT0000123_st 0.09696674
MIMAT0000124_st 0.029200554
MIMAT0004521_st -0.14371753
MIMAT0016980_st 0.044418335
MIMAT0000125_st -0.04587841
MIMAT0004522_st -0.009086132
MIMAT0000126_st 0.04189205
MIMAT0004523_st 1.1397405
MIMAT0000127_st 0.07552719
MIMAT0000128_st 0.03221798
MIMAT0000129_st 0.1678381
MIMAT0000130_st 0.08725834
MIMAT0004524_st 0.024641037
MIMAT0000131_st 0.011205673
MIMAT0016981_st 0.4542544

Total number of rows: 3163

Table truncated, full table size 81 Kbytes.

Supplementary file Size Download File type/resource
GSM2227792_miRNA4_011215H_TM6_C26.CEL.gz 775.3 Kb (ftp)(http) CEL
GSM2227792_miRNA4_011215H_TM6_C26.rma-dabg.chp.gz 308.2 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data are available on Series record

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