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Sample GSM2227790 Query DataSets for GSM2227790
Status Public on Jan 01, 2017
Title WT - 21 mon, biological rep1
Sample type RNA
Source name WT - 21 mo
Organism Mus musculus
Characteristics background strain: C57Bl/6J
genotype/variation: Wild type
tissue: Ventricular
Treatment protocol The hearts were collected and snap frozen in liquid Nitrogen.
Growth protocol The mice were collected at 4 or 21 months of age at ZT 6 ( 6 hours into the light phase)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the mIRNeasy method according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng total RNA
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Data processing The data were analyzed with GeneSpring GX Software v. 11.5 using default Affymetrix settings.
Submission date Jul 07, 2016
Last update date Jan 01, 2017
Contact name Faisal James Alibhai
Organization name University of Guelph
Department Biomedical Sciences
Lab Martino Lab
Street address 50 Stone Rd East
City Guelph
State/province ON
ZIP/Postal code N1G2W1
Country Canada
Platform ID GPL19117
Series (1)
GSE84151 miRNA Expression data from murine hearts of transgenic Clock delta 19/delta 19 mice (Clock mice) and wild type (WT) mice at 4 and 21 months of age

Data table header descriptions
VALUE Normalized signal

Data table
MIMAT0000121_st -0.0932312
MIMAT0004519_st -0.04600787
MIMAT0000122_st 0.014313698
MIMAT0004520_st 0.087703705
MIMAT0016979_st -0.33233958
MIMAT0000123_st -0.1459465
MIMAT0000124_st -0.13304615
MIMAT0004521_st -0.19359636
MIMAT0016980_st -4.25E-04
MIMAT0000125_st -0.041371346
MIMAT0004522_st 0.014606476
MIMAT0000126_st -0.03096199
MIMAT0004523_st 0.75850654
MIMAT0000127_st 0.3175001
MIMAT0000128_st 0.16534424
MIMAT0000129_st -0.016490936
MIMAT0000130_st -0.11217499
MIMAT0004524_st -0.08370972
MIMAT0000131_st 0.003174782
MIMAT0016981_st -0.59873545

Total number of rows: 3163

Table truncated, full table size 81 Kbytes.

Supplementary file Size Download File type/resource
GSM2227790_miRNA4_011215H_TM4_C24.CEL.gz 761.8 Kb (ftp)(http) CEL
GSM2227790_miRNA4_011215H_TM4_C24.rma-dabg.chp.gz 308.2 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data are available on Series record

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