GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM2227786 Query DataSets for GSM2227786
Status Public on Jan 01, 2017
Title WT - 4 mon, biological rep3
Sample type RNA
Source name WT - 4 mo
Organism Mus musculus
Characteristics background strain: C57Bl/6J
genotype/variation: Wild type
tissue: Ventricular
Treatment protocol The hearts were collected and snap frozen in liquid Nitrogen.
Growth protocol The mice were collected at 4 or 21 months of age at ZT 6 ( 6 hours into the light phase)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the miRNeasy method according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng total RNA
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Data processing The data were analyzed with GeneSpring GX Software v. 11.5 using default Affymetrix settings.
Submission date Jul 07, 2016
Last update date Jan 01, 2017
Contact name Faisal James Alibhai
Organization name University of Guelph
Department Biomedical Sciences
Lab Martino Lab
Street address 50 Stone Rd East
City Guelph
State/province ON
ZIP/Postal code N1G2W1
Country Canada
Platform ID GPL19117
Series (1)
GSE84151 miRNA Expression data from murine hearts of transgenic Clock delta 19/delta 19 mice (Clock mice) and wild type (WT) mice at 4 and 21 months of age

Data table header descriptions
VALUE Normalized signal

Data table
MIMAT0000121_st 0.1769867
MIMAT0004519_st -0.21437883
MIMAT0000122_st -0.008853912
MIMAT0004520_st -0.20515752
MIMAT0016979_st 0.10583019
MIMAT0000123_st 0.14405155
MIMAT0000124_st 0.06795025
MIMAT0004521_st -0.11979926
MIMAT0016980_st 4.24E-04
MIMAT0000125_st -0.006678581
MIMAT0004522_st -0.024216175
MIMAT0000126_st -0.082862854
MIMAT0004523_st -0.3963058
MIMAT0000127_st 0.20256662
MIMAT0000128_st 0.008734703
MIMAT0000129_st 0.16633224
MIMAT0000130_st 0.001742363
MIMAT0004524_st -0.024641514
MIMAT0000131_st -0.12748337
MIMAT0016981_st 0.9769082

Total number of rows: 3163

Table truncated, full table size 81 Kbytes.

Supplementary file Size Download File type/resource
GSM2227786_miRNA4_011215H_TM12_C64.CEL.gz 765.8 Kb (ftp)(http) CEL
GSM2227786_miRNA4_011215H_TM12_C64.rma-dabg.chp.gz 308.5 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap