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Sample GSM2227781 Query DataSets for GSM2227781
Status Public on Jan 01, 2017
Title Clock Mutant - 4 mon, biological rep1
Sample type RNA
Source name Clock - 4 mo
Organism Mus musculus
Characteristics background strain: C57Bl/6J
genotype/variation: Clock delta 19/delta 19
tissue: ventricular
Treatment protocol The hearts were collected and snap frozen in liquid Nitrogen.
Growth protocol The mice were collected at 4 or 21 months of age at ZT 6 ( 6 hours into the light phase)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the miRNeasy method according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500ng total RNA
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Data processing The data were analyzed with GeneSpring GX Software v. 11.5 using default Affymetrix settings.
Submission date Jul 07, 2016
Last update date Jan 01, 2017
Contact name Faisal James Alibhai
Organization name University of Guelph
Department Biomedical Sciences
Lab Martino Lab
Street address 50 Stone Rd East
City Guelph
State/province ON
ZIP/Postal code N1G2W1
Country Canada
Platform ID GPL19117
Series (1)
GSE84151 miRNA Expression data from murine hearts of transgenic Clock delta 19/delta 19 mice (Clock mice) and wild type (WT) mice at 4 and 21 months of age

Data table header descriptions
VALUE Normalized signal

Data table
MIMAT0000121_st 0.002337456
MIMAT0004519_st 0.28200412
MIMAT0000122_st 0.13508797
MIMAT0004520_st 0.5657549
MIMAT0016979_st 0.01738143
MIMAT0000123_st -0.03873253
MIMAT0000124_st -0.13944626
MIMAT0004521_st -0.09688771
MIMAT0016980_st 0.38871384
MIMAT0000125_st -0.024220467
MIMAT0004522_st 0.32023954
MIMAT0000126_st 0.012626648
MIMAT0004523_st -0.03714347
MIMAT0000127_st -0.26933956
MIMAT0000128_st -0.27835846
MIMAT0000129_st 0.016490936
MIMAT0000130_st -0.003772736
MIMAT0004524_st 0.3012519
MIMAT0000131_st 0.07200432
MIMAT0016981_st -0.055081844

Total number of rows: 3163

Table truncated, full table size 81 Kbytes.

Supplementary file Size Download File type/resource
GSM2227781_miRNA4_011215H_TM7_A74.CEL.gz 771.4 Kb (ftp)(http) CEL
GSM2227781_miRNA4_011215H_TM7_A74.rma-dabg.chp.gz 308.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data are available on Series record

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