|
| Status |
Public on Aug 10, 2017 |
| Title |
Genomc DNA from 293T-TET2-RUNX1 |
| Sample type |
genomic |
| |
|
| Source name |
293T-TET2-RUNX1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 293T
gender: Female
tissue: Kidney
developmental stage: Embryo
transduced with: TET2 and RUNX1
|
| Treatment protocol |
293T cells were transduced genes of interest by using lentivirus vector system. Transduced cells were purified by antibiotics
|
| Growth protocol |
293T cells were provided from RIKEN Bioresource Center (BRC) and were cultured in Dulbecco's modified Eagle's medium (DMEM) (Wako), supplemented with 10% FBS and penicillin/streptomycin (100 U/ml, 100 µg/ml) (Life Technologies) on Poly-D-lysine courted dish (Becton Dickinson).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified using NucleoSpin Tissue (Macherey-Nagel) according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450k Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
TET2 and RUNX1 transduced
|
| Data processing |
Signal intensities were measured by GenomeStudio software v2010.3. Color balance adjustment, quantile normalization and M-value calculation were done by lumi, a bioconductor package.
|
| |
|
| Submission date |
Jul 07, 2016 |
| Last update date |
Aug 10, 2017 |
| Contact name |
Takahiro Suzuki |
| E-mail(s) |
takahiro.suzuki.aa@riken.jp
|
| Phone |
81-45-503-9222
|
| Organization name |
RIKEN
|
| Department |
Center for Integrative Medical Sciences
|
| Lab |
Laboratory for Cellular Function Conversion Technologies
|
| Street address |
1-7-22 Suehiro-cho, Tsurumi-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE60316 |
RUNX1-Mediated Binding Site Directed DNA Demethylation is a Novel Mechanism of Hematopoietic Gene Activation. |
|
