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| Status |
Public on Aug 11, 2016 |
| Title |
vec_3 |
| Sample type |
SRA |
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| Source name |
MPRA vector
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| Organism |
synthetic construct |
| Characteristics |
tissue type: MPRA vector
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| Extracted molecule |
other |
| Extraction protocol |
C2C12 cells were harvested in TRIzol.
Libraries were generated from cDNA using indexed primers against the tag-containing GFP.
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| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 2500 |
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| Description |
Vector DNA
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| Data processing |
Casava 1.8.2 was used to call bases
Reads containing barcodes with a perfect match to a designed barcode and originating from a read with GFP sequence were counted.
analysis scripts were run in R, and are provided in supplemental material for the paper
Supplementary_files_format_and_content: a tab-delimited processed file showing normalized counts for tags associated to lincp21 genomic sequence and all relevent annotation information
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| Submission date |
Jun 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Abbie Groff |
| E-mail(s) |
agroff@fas.harvard.edu
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| Organization name |
Harvard University
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| Department |
Stem Cell and Regenerative Biology;
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| Lab |
John Rinn
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| Street address |
7 Divinity Ave
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platform ID |
GPL19604 |
| Series (1) |
| GSE73472 |
In vivo characterization of Linc-p21 reveals functional cis-regulatory DNA elements |
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| Relations |
| BioSample |
SAMN05294027 |
| SRA |
SRX1879451 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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