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Sample GSM2209815 Query DataSets for GSM2209815
Status Public on Jan 01, 2017
Title Vector-1
Sample type SRA
Source name HepG2.2.15-vector cell
Organism Homo sapiens
Characteristics cell line: HepG2.2.15
lentivirus: vector
Treatment protocol CD36-overexpression-lentivirus and Vector-lentivirus were transfected into HepG2.2.15 cells.
Growth protocol Modified Eagle Medium (MEM) containing 10% fetal calf serum and 500g of G418/ml.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cells by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Agilent) and kept at -80℃. The RNA with RIN >8.0 is right for cDNA library construction.
Ion Proton Sequencing Protocol.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Ion Torrent Proton
Description Processed data file: All_Counts.txt
Data processing Base calling using Torrent Suite Software and Torrent Server.
Reads filtering under criteria removing reads with 20% of the base quality lower than 13.
Mapsplice v2.1.8 mapping to reference genome.
Reads counting using HTSeq.
Genome_build: hg19 (GRCh37)
Supplementary_files_format_and_content: All_Counts.txt: Tab-delimited text file contains raw counts for each sample.
Submission date Jun 21, 2016
Last update date May 15, 2019
Contact name 健 黄
Phone 15086067610
Organization name 遵义医学院附属医院
Street address 汇川区大连路149号遵义医学院附属医院
City 遵义市
State/province 贵州省
ZIP/Postal code 563003
Country China
Platform ID GPL17303
Series (1)
GSE83577 Genome-wide Transcriptome Analysis of CD36 Overexpression in HepG2.2.15 Cells to Explore Its Regulation Role of Metabolism and HBV Life Cycle
BioSample SAMN05277752
SRA SRX1868801

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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