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Sample GSM2193105 Query DataSets for GSM2193105
Status Public on Jun 09, 2016
Title Sertoli cells, 9d, 2
Sample type RNA
 
Source name 9-day-old Sertoli cell
Organism Rattus norvegicus
Characteristics tissue: testis
Treatment protocol After primary culture for another 24 hours, Sertoli cells were divided into 2 groups: control and 0.1mM MBP. After 24 hours culture, cells were placed on ice and treated with Trizol Reagent.
Growth protocol Sertoli cells were isolated and cultured in DMEM/F12 medium.Testes from rats were aseptically removed, decapsulated, and digested into cell suspension. Isolated cells were placed on a tissue cultured dish in DMEM/F12 supplemented with 10% fetal bovine serum.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Genechips were scanned on the 3000 7G, an Affymetrix GeneChip scanner
Description microRNA expression data from 9-day-old rat Sertoli cells.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Jun 08, 2016
Last update date Jun 09, 2016
Contact name Xiaoqin Yin
E-mail(s) yinxiaoqin1991@126.com
Organization name School of medicine, Shanghai Jiao Tong University
Department Shanghai Children’s Hospital
Lab Department of endocrinology
Street address No. 355 Luding Road, Shanghai 200062, P.R.China.
City Shanghai
State/province Shanghai
ZIP/Postal code 200062
Country China
 
Platform ID GPL19117
Series (1)
GSE83106 Expression data from 9-day-old rat Sertoli cells and MBP-treated rat Sertoli cells

Data table header descriptions
ID_REF
VALUE RMA signal
2C_(miRNA-4_0).rma-dabg-Detection

Data table
ID_REF VALUE 2C_(miRNA-4_0).rma-dabg-Detection
MIMAT0001619_st 4.295431 P
MIMAT0000547_st 7.150113 P
MIMAT0004637_st 0.7256527 A
MIMAT0000550_st 1.566623 P
MIMAT0017026_st 0.1090386 A
MIMAT0000552_st 2.56239 P
MIMAT0000553_st 8.261045 P
MIMAT0000554_st 7.760418 P
MIMAT0000557_st 0.4601056 A
MIMAT0004639_st 0.4028227 A
MIMAT0017028_st 1.011503 A
MIMAT0000560_st 2.127428 P
MIMAT0000561_st 2.922666 P
MIMAT0000562_st 13.11564 P
MIMAT0000563_st 5.434533 P
MIMAT0017029_st 6.56678 P
MIMAT0000564_st 6.352797 P
MIMAT0017031_st 2.551028 P
MIMAT0000566_st 7.58173 P
MIMAT0004641_st 1.064514 A

Total number of rows: 1277

Table truncated, full table size 33 Kbytes.




Supplementary file Size Download File type/resource
GSM2193105_2C_miRNA-4_0_.CEL.gz 738.4 Kb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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