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Sample GSM2181547 Query DataSets for GSM2181547
Status Public on May 31, 2018
Title T24 cells_control siRNA_rep2
Sample type RNA
 
Source name human bladder cancer cells T24, transfected with control siRNA
Organism Homo sapiens
Characteristics cell type: T24
transfection: control siRNA
Treatment protocol T24 cells were transfected with 20μM siRNA control (siNC) or siRNA NF90 (siNF90) for 48 hours using Lipofectamine 3000. Then, cells were washed with PBS and placed on ice in the Trizol solution.
Growth protocol Cells were maintained in RPMI1640 supplemented with 10% FBS, 100 units/ml penicillin and 100 μg/ml streptomycin at 37 “C.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Per RNA sample, 1 ug were used as input into the procedure as recommended by protocol (Genisphere FlashTagTM Biotin RNA Labeling for Affymetrix GeneChip miRNA arrays).
 
Hybridization protocol Labeled miRNA was hybridized to the array for 16 hours at 48 “C and 60 rpm as described in the protocol.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G
Description human bladder cancer cells T24, transfected with control siRNA
Data processing The data were analyzed with Robust Multi-chip Average (RMA) algorithm as normalization method.
 
Submission date May 30, 2016
Last update date May 31, 2018
Contact name Junlong Zhuang
E-mail(s) zhuangjl-2008@163.com
Organization name Institute of Urology, Drum Tower hospital
Street address 321# Zhongshan Road
City Nanjing
ZIP/Postal code 210008
Country China
 
Platform ID GPL19117
Series (1)
GSE82024 Expression data from human T24 cells transfected with siNC or siNF90

Data table header descriptions
ID_REF
VALUE RMA normalized log2 (expression values)

Data table
ID_REF VALUE
14q0_st 0.254658
14qI-1_st 0.904015
14qI-1_x_st 0.887983
14qI-2_st 0.391766
14qI-3_x_st 0.303478
14qI-4_st 0.126677
14qI-4_x_st 0.408192
14qI-5_st 0.308316
14qI-6_st 0.303478
14qI-7_st 0.747311
14qI-8_st 0.4405
14qI-8_x_st 0.473312
14qI-9_x_st 0.316262
14qII-10_st 0.587572
14qII-11_st 0.494466
14qII-12_st 0.163458
14qII-12_x_st 0.500925
14qII-13_st 1.049776
14qII-14_st 0.469859
14qII-14_x_st 0.146487

Total number of rows: 6658

Table truncated, full table size 160 Kbytes.




Supplementary file Size Download File type/resource
GSM2181547_UBC_T24_SiNC_2_miRNA-4_0_.CEL.gz 697.1 Kb (ftp)(http) CEL
Processed data included within Sample table

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