|
| Status |
Public on Aug 31, 2016 |
| Title |
Ovol2_GE_02 |
| Sample type |
RNA |
| |
|
| Source name |
mESC, J1 in 2i, replicate 2
|
| Organism |
Mus musculus |
| Characteristics |
cell types: stem cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted from samples using an AllPrep DNA/RNA Micro Kit (Qiagen, Hilden, Germany). The quality of RNA samples was checked using an Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
Twenty five nanogram of total RNA was labeled with Cy3-CTP with a Quick Amp Labeling Kit (Agilent Technologies). Dye incorporation and cRNA yield were checked with the NanoDrop Spectrophotometer.
|
| |
|
| Hybridization protocol |
One ug of Cy3-labelled cRNA was fragmented following the manufacturers instructions. On completion of the fragmentation reaction, 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent SurePrint G3 Mouse GE 8x60K Microarray (G4852A) for 16hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then immediately subjected to a microarray scanner.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner) using one color scan setting for 8x60k array slides ( Scan resolution 5um).
|
| Description |
Mouse ES cells_J1_cultured in 2i_rep2
|
| Data processing |
Agilent Feature Extraction and GeneSpring software was used for data processing.
|
| |
|
| Submission date |
May 28, 2016 |
| Last update date |
Aug 31, 2016 |
| Contact name |
Kuniya Abe |
| E-mail(s) |
tail@rtc.riken.jp
|
| Phone |
+81-29-836-9198
|
| Organization name |
RIKEN
|
| Department |
BioResource Center
|
| Lab |
Mammalian Genome Dynamics
|
| Street address |
3-1-1 Koyadai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-0074 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
|
