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Sample GSM2178980 Query DataSets for GSM2178980
Status Public on Nov 15, 2016
Title LMP2_CD8ab_rep3
Sample type SRA
Source name CD8αβ T cells
Organism Homo sapiens
Characteristics cell type: CD8ab
cell derivation: Regenerated from LMP2 T-iPSCs
stimulation method: LCL pulsed with LMP2 peptide with IL-7 and IL-21
days after stimulation: 7
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using an RNeasy Plus Mini Kit (QIAGEN)
Libraries for RNA-sequence were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs).
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
Data processing Basecalls performed using CASAVA
Sequence alignment performed using our in-house pipelines (Genomon Fusion).
Fragments per kilobase of exon per million fragments mapped (FPKM) was calculated for Refseq genes and then log-transformed.
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample
Submission date May 27, 2016
Last update date May 15, 2019
Contact name Takuya Maeda
Phone 81-75-751-3818
Organization name Institute for Frontier Medical Science, Kyoto University
Department Department of Immunology
Street address 53 Kawahara-cho Shogoin Sakyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 606-8507
Country Japan
Platform ID GPL21290
Series (1)
GSE81975 Gene expression profile of regenerated CD8αα T cells and CD8αβ T cells from LMP2 T-iPSCs
BioSample SAMN05180912
SRA SRX1802837

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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