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Sample GSM2174922 Query DataSets for GSM2174922
Status Public on Oct 23, 2018
Title U14_BS
Sample type SRA
Source name normal mouse U14 cells - bisulfite-treated mRNAs
Organism Mus musculus
Characteristics cell line: U14
cell type: Uterine Cervix Cancer
Extracted molecule total RNA
Extraction protocol mRNAs of of human and mouse cell lines were purified using oligo (dT)-conjugated magnetic beads followed by RiboMinus treatement.
Bisulfite treated mRNAs were reverse transcribed to cDNA using ACT random hexamer primers. The cDNAs were subjected to libraries construction using KAPA Stranded mRNA-Seq Kit (KAPA)
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina HiSeq 2000
Description bisulfite treated mRNA
Mouse Uterine Cervix Cancer
Data processing Raw reads for each sample were stripped of adaptor sequences and low quality bases were removed using Trimmomatic. Filtered paired-end reads were mapped against human (hg19) or mouse (mm10) reference genome by Bismark.
The methylated sites were called by Bismark (
Genome_build: hg19 or mm10
Supplementary_files_format_and_content: supplementary file includes the methylated sites
Submission date May 23, 2016
Last update date May 15, 2019
Contact name Bao-Fa Sun
Organization name Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
Street address Da-Tun Road
City Beijing
ZIP/Postal code 100101
Country China
Platform ID GPL13112
Series (1)
GSE74771 Single-base resolution methylome of 5-methylcytosine in mammalian transcriptome
BioSample SAMN05163475
SRA SRX1792779

Supplementary file Size Download File type/resource
GSM2174922_U14_BS.txt.gz 103.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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