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Sample GSM2174895 Query DataSets for GSM2174895
Status Public on May 24, 2016
Title PPI-005 BM_last
Sample type genomic
 
Source name last tumor
Organism Homo sapiens
Characteristics subject status: myelodysplastic syndromes (MDS) patient
subject id: PPI-005
tissue type: bone marrow tumor
time point: at the end of treatment
Treatment protocol panobinostat plus decitabine on the EOT (end of treatment) samples.  Pre-study = none.
Growth protocol none
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted from mononuclear cell preparations of leukemic blasts obtained from peripheral blood or bone marrow at diagnosis using the Qiagen DNA Blood Mini Kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, PicoGreen and agarose gel electrophoresis.
Label biotin
Label protocol As per manufacturer (Affymetrix)
 
Hybridization protocol DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
Scan protocol The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
Description 1321517; 22924.CEL
Data processing Copy number analysis from the SNP arrays was performed using Partek Genomics Suite (Partek, Inc),
 
Submission date May 23, 2016
Last update date May 24, 2016
Contact name Matt Walter
E-mail(s) mjwalter@dom.wustl.edu
Organization name washington university in st.louis school of medicine
Department oncology
Street address 4940 parkview
City st.louis
ZIP/Postal code 63110
Country USA
 
Platform ID GPL6801
Series (1)
GSE81738 Affymetrix SNP array data for myelodysplastic syndromes samples

Supplementary file Size Download File type/resource
GSM2174895_22924.CEL.gz 29.5 Mb (ftp)(http) CEL
Processed data are available on Series record

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