|
Status |
Public on May 20, 2016 |
Title |
Primary Mouse_SQ1 treated_Replicate6 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
primary hepatocytes_untreated control
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl/J gender: male cell type: Primary cultured Mouse Hepatocytes treated with: none (untreated control)
|
Treatment protocol |
48 hours after plating, hepatocytes were left untreated or treated with 100 µM SQ1 or 30 µM pravastatin for 48 hours.
|
Growth protocol |
Primary hepatocytes plated at density and maintained in Williams Medium E.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Qiagen lysis buffer following manufacturer's instructions
|
Label |
A647
|
Label protocol |
500ng of total RNA was input to the TargetAMP 1-Round Aminoallyl-aRNA Amplification Kit 101 (Epicentre, Madison, WI) to produce aminoallyl-aRNA according to the vendor’s protocol. Five ug of each aminoallyl-aRNA sample was labeled with Alexa 555 or Alexa 647 (Molecular probes/ Life Technologies, Foster City, CA).
|
|
|
Channel 2 |
Source name |
primary hepatocytes_SQ1
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl/J gender: male cell type: Primary cultured Mouse Hepatocytes treated with: SQ1
|
Treatment protocol |
48 hours after plating, hepatocytes were left untreated or treated with 100 µM SQ1 or 30 µM pravastatin for 48 hours.
|
Growth protocol |
Primary hepatocytes plated at density and maintained in Williams Medium E.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Qiagen lysis buffer following manufacturer's instructions
|
Label |
A555
|
Label protocol |
500ng of total RNA was input to the TargetAMP 1-Round Aminoallyl-aRNA Amplification Kit 101 (Epicentre, Madison, WI) to produce aminoallyl-aRNA according to the vendor’s protocol. Five ug of each aminoallyl-aRNA sample was labeled with Alexa 555 or Alexa 647 (Molecular probes/ Life Technologies, Foster City, CA).
|
|
|
|
Hybridization protocol |
0.825µg of Alexa 555 labeled Aminoallyl-aRNA and 0.825µg of Alexa 647 labeled Aminoallyl-aRNA were mixed together and allowed to co-hybridize on the array for 17 hours at 65°C at 10rpm in the hybridization rotator. After hybridization the slide was washed with Agilent GE Wash Buffers following the Agilent’s protocol.
|
Scan protocol |
Scanned on an Agilent G2565AA scanner. Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
Description |
Mo SQ1_2
|
Data processing |
Agilent Feature Extraction Software was used for background subtraction and LOWESS normalization.
|
|
|
Submission date |
May 19, 2016 |
Last update date |
May 20, 2016 |
Contact name |
Thomas Kocarek |
E-mail(s) |
t.kocarek@wayne.edu
|
Phone |
313-577-6580
|
Organization name |
Wayne State University
|
Department |
Institute of Environmental Health Sciences
|
Street address |
6135 Woodward Ave
|
City |
Detroit |
State/province |
MI |
ZIP/Postal code |
48202 |
Country |
USA |
|
|
Platform ID |
GPL11202 |
Series (2) |
GSE81658 |
Mouse Primary Cultured Hepatocytes: Control vs SQ1 or Pravastatin treatment. |
GSE81659 |
Expression data from squalestatin 1- or pravastatin-treated primary cultured mouse and rat hepatocytes. |
|