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Sample GSM2157634 Query DataSets for GSM2157634
Status Public on May 18, 2016
Title EndoC T-Ag 460 GFP
Sample type SRA
Source name Endo-ßH1C cell line (pancreas), T-Ag siRNA 460, GFP expression
Organism Homo sapiens
Characteristics cell line: Endo-beta-H1C
cell type: pancreatic beta-cell line
gain of function: GFP
sirna treatment: T-Antigen siRNA 460
Extracted molecule total RNA
Extraction protocol ABI picopure RNA extraction kit.
KAPA stranded RNA-Seq kit.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Description RSq48
Processed data file: normalizedCounts_endoC.txt
Data processing Raw reads were mapped to hg19 transcriptome using STAR version
A custom script was used to count transcripts.
Genome_build: hg19 transcriptome, aggregated from UCSC, RefSeq and GENCODE v12 databases.
Supplementary_files_format_and_content: normalizedCounts_endoC.txt: The processed data file contains 9 tab-delimited columns: the first column is gene symbol, and columns that come after are DESeq normalized counts of the respective samples. We used the DESeq (Huber et al. [PMID: 20979621]) normalization.
Submission date May 18, 2016
Last update date May 15, 2019
Contact name Seung Kim
Organization name Stanford University
Department Developmental Biology
Lab Seung Kim Lab
Street address 279 Campus Dr B303
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platform ID GPL18573
Series (2)
GSE57973 Age-dependent gene expression changes in human islets
GSE79469 Age-dependent pancreatic gene regulation reveals mechanisms governing human β-cell function
BioSample SAMN05011595
SRA SRX1775525

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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