|Public on May 28, 2018
|H3122 lung cancer cell line
|cell line: H3122
|Standard DNA extraction protocol using Qiagen prep kit.
Libraries were prepared according to Illumina's instructions.
|Illumina NextSeq 500
|Base calling performed using CASAVA version 1.7
The sequencing reads were aligned to the reference sequence (hg 19) using the Bowtie2 (v.2.2.2) software.
Finding, estimation and annotation of ChIP-seq peaks were carried out by the Homer (v.4.8.2) platform.
ChIP-fragment density at each position in the genome was estimated by starting with each tag and extending it by the estimated fragment length determined by autocorrelation.
The ChIP-fragment density is then defined as the total number of overlapping fragments at each position in the genome.
Supplementary_files_format_and_content: bedGraph files were generated by makeUCSCfile module in the Hommer software. Scores represent ChIP-seq density value as described in data processing step.
|May 16, 2016
|Last update date
|May 15, 2019
|Korea Research Institutue of Bioscience & Biotechnology
|Personalized Genomic Medicine Research Center
|125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Korea
|ChIP-seq for identifying molecules associated with epithelial-mesenchymal transition and acquired resistance to ALK inhibitors
|Epithelial-mesenchymal transition and acquired resistance to ALK inhibitors