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Sample GSM2152841 Query DataSets for GSM2152841
Status Public on May 09, 2017
Title WildType Rep2
Sample type SRA
 
Source name WT_fetal liver CD19+B220+
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: WT; Wildtype
developmental stage: Embryonic day 17.5 (E17.5)
tissue: liver
cell type: CD19+B220+ cells
Extracted molecule total RNA
Extraction protocol Fetal Livers were dissected from E17.5 embryos for each genotype and stained for surface markers CD19 and B220. The cells positive for both CD19 and B220 were sorted and RNA was extracted from these cells.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description WT2
Data processing Base Calling was done using RTA v(2.4.1.1)
Alignment was done using TopHat v(2.0.7)
Peak Calling was performed using Picard
The Bioconductor package DESeq2 was used to generate normalised estimates of transcript abundance
Genome_build: mm10
Supplementary_files_format_and_content: comma separated file including RPKM values for each Sample.
 
Submission date May 16, 2016
Last update date May 15, 2019
Contact name Tessa Crompton
Organization name UCL
Department Immunobiology
Street address 30 Guilford Street
City London
ZIP/Postal code WC1N 1EH
Country United Kingdom
 
Platform ID GPL19057
Series (1)
GSE81467 The transcription factor Gli3 promotes B cell development in the fetal liver through repression of Shh
Relations
BioSample SAMN05001442
SRA SRX1763668

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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